Neuroprotection of rapamycin in lactacystin-induced neurodegeneration via autophagy enhancement

Tianhong Pan, Seiji Kondo, Wen Zhu, Wenjie Xie, Joseph Jankovic, Weidong Le

Research output: Contribution to journalArticle

174 Scopus citations

Abstract

The ubiquitin-proteasome system (UPS) and the autophagy-lysosomal pathway (ALP) are the two most important cellular mechanisms for protein degradation. To investigate the role of autophagy in reversing neuronal injury, the proteasome inhibitor lactacystin was used to cause UPS dysfunction in differentiated PC12 cells and in C57BL/6 mice and rapamycin was used as an autophagy enhancer. The results showed that rapamycin pre-treatment attenuated lactacystin-induced apoptosis and reduced lactacystin-induced ubiquitinated protein aggregation in differentiated PC12 cells. The observed protection was partially blocked by the autophagy inhibitor 3-methyladenine. Furthermore, post-treatment of mice with rapamycin significantly attenuated lactacystin-induced loss of nigral DA neurons and the reduction of striatal DA levels. The lactacystin-induced high molecular ubiquitinated proteins were also attenuated by rapamycin treatment in vivo. In addition, as a chemical compound, rapamycin caused an increase of bcl2 protein level and blocked the release of cytochrome c from mitochondria to cytosal. We concluded that the neuroprotective effect of rapamycin is partially mediated by autophagy enhancement through enhanced degradation of misfolded proteins and autophagy enhancement may be considered to be a promising strategy to prevent diseases associated with misfolded/aggregated proteins, such as Parkinson's disease.

Original languageEnglish (US)
Pages (from-to)16-25
Number of pages10
JournalNeurobiology of Disease
Volume32
Issue number1
DOIs
StatePublished - Oct 2008

Keywords

  • Autophagy
  • Lactacystin
  • Parkinson's disease
  • Rapamycin
  • Ubiquitin-proteasome system

ASJC Scopus subject areas

  • Neurology

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