TY - JOUR
T1 - Neuronal differentiation of NTE-deficient embryonic stem cells
AU - Li, Zhongyou
AU - Szurek, Paul F.
AU - Jiang, Chuantao
AU - Pao, Annie
AU - Bundy, Brian
AU - Le, Weidong
AU - Bradley, Allan
AU - Yu, Y. Eugene
N1 - Funding Information:
We thank Y. Qi, S. Rivera, G. Schuster, S. Vaishnav, J. Wesley, and B. Rodwin for technical assistance, R. Behringer and P. Soriano for DNA constructs, and E. VanDette and A. Bakin for assistance in immunofluorescence analysis. This work was supported by the grants from NIH (to A.B.) and from Roswell Park Cancer Institute (to Y.Y.). Part of this research utilized core facilities supported partially by RPCI’s NCI-funded Cancer Center Support Grant, CA16056.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005/5/20
Y1 - 2005/5/20
N2 - Organophosphates induce neurological disorders. One of the enzymes inhibited by these compounds is neuropathy target esterase (NTE). In vitro, inhibition of NTE activity by organophosphates is correlated with inhibition of neurite initiation and reduction of neurite length, supporting the hypothesis that organophosphate-induced neurological disorders are caused by inhibition of NTE activity. However, there is no direct evidence for the involvement of NTE in organophosphate-induced impairment of neurites in vitro. To examine the role of NTE, we have generated NTE-deficient mouse embryonic stem cells. These cells can differentiate into neuron-like cells. Although NTE-deficient cells exhibited a delay in neurite initiation in vitro, both the proportion of neuron-like cells which initiated neurites and the elongation of these neurites occurred at the normal rate. These results demonstrate that NTE activity is not required for neurite initiation or elongation per se, but is essential for the optimal rate of neurite initiation.
AB - Organophosphates induce neurological disorders. One of the enzymes inhibited by these compounds is neuropathy target esterase (NTE). In vitro, inhibition of NTE activity by organophosphates is correlated with inhibition of neurite initiation and reduction of neurite length, supporting the hypothesis that organophosphate-induced neurological disorders are caused by inhibition of NTE activity. However, there is no direct evidence for the involvement of NTE in organophosphate-induced impairment of neurites in vitro. To examine the role of NTE, we have generated NTE-deficient mouse embryonic stem cells. These cells can differentiate into neuron-like cells. Although NTE-deficient cells exhibited a delay in neurite initiation in vitro, both the proportion of neuron-like cells which initiated neurites and the elongation of these neurites occurred at the normal rate. These results demonstrate that NTE activity is not required for neurite initiation or elongation per se, but is essential for the optimal rate of neurite initiation.
KW - Embryonic stem cells
KW - In vitro differentiation
KW - Neurite initiation/elongation
KW - Neuropathy target esterase
UR - http://www.scopus.com/inward/record.url?scp=17044430597&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17044430597&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.03.090
DO - 10.1016/j.bbrc.2005.03.090
M3 - Article
C2 - 15823557
AN - SCOPUS:17044430597
VL - 330
SP - 1103
EP - 1109
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -