Near-Infrared Light-Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification

Hongqian Chu, Jian Zhao, Yongsheng Mi, Yuliang Zhao, Lele Li

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.

Original languageEnglish (US)
Pages (from-to)14877-14881
Number of pages5
JournalAngewandte Chemie - International Edition
Volume58
Issue number42
DOIs
StatePublished - Oct 14 2019

Keywords

  • RNA
  • bioimaging
  • fluorescence
  • hybridization chain reaction
  • signal amplification

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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