TY - JOUR
T1 - Near-Infrared Light-Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification
AU - Chu, Hongqian
AU - Zhao, Jian
AU - Mi, Yongsheng
AU - Zhao, Yuliang
AU - Li, Lele
N1 - Funding Information:
This research was financially supported by the National Natural Science Foundation of China (Nos. 21822401, 21771044) and the Young Thousand Talents Program.
Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/10/14
Y1 - 2019/10/14
N2 - Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.
AB - Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.
KW - RNA
KW - bioimaging
KW - fluorescence
KW - hybridization chain reaction
KW - signal amplification
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U2 - 10.1002/anie.201906224
DO - 10.1002/anie.201906224
M3 - Article
C2 - 31373118
AN - SCOPUS:85071027735
VL - 58
SP - 14877
EP - 14881
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 42
ER -