Near-Infrared Light-Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification

Hongqian Chu, Jian Zhao, Yongsheng Mi, Yuliang Zhao, Lele Li

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.

Original languageEnglish (US)
Pages (from-to)14877-14881
Number of pages5
JournalAngewandte Chemie - International Edition
Volume58
Issue number42
DOIs
StatePublished - Oct 14 2019

Keywords

  • RNA
  • bioimaging
  • fluorescence
  • hybridization chain reaction
  • signal amplification

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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