Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Marco Gaspari, Mark Ming Cheng Cheng, Rosa Terracciano, Xuewu Liu, A. Jasper Nijdam, Lisa Vaccari, Enzo Di Fabrizio, Emanuel F. Petricoin, Lance A. Liotta, Giovanni Cuda, Salvatore Venuta, Mauro Ferrari

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

Original languageEnglish (US)
Pages (from-to)1261-1266
Number of pages6
JournalJournal of Proteome Research
Volume5
Issue number5
DOIs
StatePublished - May 1 2006

Keywords

  • Human plasma
  • MALDI-TOF
  • Mass spectrometry
  • Nanoporous silica
  • Nanoporous silicon
  • Peptide profiling

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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