Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Marco Gaspari; Mark Ming Cheng Cheng; Rosa Terracciano; Xuewu Liu; A. Jasper Nijdam; Lisa Vaccari; Enzo Di Fabrizio; Emanuel F. Petricoin; Lance A. Liotta; Giovanni Cuda; Salvatore Venuta; Mauro Ferrari

doi:10.1021/pr050417+

Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Marco Gaspari, Mark Ming Cheng Cheng, Rosa Terracciano, Xuewu Liu, A. Jasper Nijdam, Lisa Vaccari, Enzo Di Fabrizio, Emanuel F. Petricoin, Lance A. Liotta, Giovanni Cuda, Salvatore Venuta, Mauro Ferrari

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

Original language	English (US)
Pages (from-to)	1261-1266
Number of pages	6
Journal	Journal of Proteome Research
Volume	5
Issue number	5
DOIs	https://doi.org/10.1021/pr050417+
State	Published - May 1 2006

Keywords

Human plasma
MALDI-TOF
Mass spectrometry
Nanoporous silica
Nanoporous silicon
Peptide profiling

ASJC Scopus subject areas

Genetics
Biotechnology
Biochemistry

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10.1021/pr050417+

Cite this

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title = "Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma",

abstract = "Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.",

keywords = "Human plasma, MALDI-TOF, Mass spectrometry, Nanoporous silica, Nanoporous silicon, Peptide profiling",

author = "Marco Gaspari and Cheng, {Mark Ming Cheng} and Rosa Terracciano and Xuewu Liu and Nijdam, {A. Jasper} and Lisa Vaccari and {Di Fabrizio}, Enzo and Petricoin, {Emanuel F.} and Liotta, {Lance A.} and Giovanni Cuda and Salvatore Venuta and Mauro Ferrari",

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AU - Gaspari, Marco

AU - Cheng, Mark Ming Cheng

AU - Terracciano, Rosa

AU - Liu, Xuewu

AU - Nijdam, A. Jasper

AU - Vaccari, Lisa

AU - Di Fabrizio, Enzo

AU - Petricoin, Emanuel F.

AU - Liotta, Lance A.

AU - Cuda, Giovanni

AU - Venuta, Salvatore

AU - Ferrari, Mauro

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

AB - Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

KW - Human plasma

KW - MALDI-TOF

KW - Mass spectrometry

KW - Nanoporous silica

KW - Nanoporous silicon

KW - Peptide profiling

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Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Abstract

Keywords

ASJC Scopus subject areas

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