Nanoporous scaffold with enzymes encapsulated during flow induced gelation for efficient H2O2 biosensing

Donglai Lu, Joshua Cardiel, Guozhong Cao, Amy Q. Shen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel and versatile processing method was developed for the formation of nanoporous scaffold with in-situ enzyme immobilization for efficient biosensor applications. Our new approach used microfluidic devices to facilitate the single throughput, in-situ process to immobilize enzyme in a nanoporous scaffold via flow induced gelation, under ambient conditions. The nanoporous gel serves as a favorable host matrix for the immobilization of horseradish peroxidase (HRP) and ferrocene methanol (FcMeOH). The biosensor designed by the nanoporous scaffold demonstrated a highly linear amperometric response over the 0.1-1.5 mM range of H2O2 examined, with high sensitivity of 2.5 μM, high stability and selectivity, and good precision (RSD = 2.4%). This flow induced immobilziation technique opens up new pathways for designing simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site testing of a variety of biomolecules.

Original languageEnglish (US)
Title of host publication10AIChE - 2010 AIChE Annual Meeting, Conference Proceedings
StatePublished - Dec 1 2010
Event2010 AIChE Annual Meeting, 10AIChE - Salt Lake City, UT, United States
Duration: Nov 7 2010Nov 12 2010

Other

Other2010 AIChE Annual Meeting, 10AIChE
CountryUnited States
CitySalt Lake City, UT
Period11/7/1011/12/10

Keywords

  • Electrochemical sensor
  • Enzyme
  • Flow induced gelation
  • Immobilization
  • Microfluidics
  • Nanoporous scaffolds

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Nanoporous scaffold with enzymes encapsulated during flow induced gelation for efficient H<sub>2</sub>O<sub>2</sub> biosensing'. Together they form a unique fingerprint.

Cite this