Abstract
A novel and versatile processing method was developed for the formation of nanoporous scaffold with in-situ enzyme immobilization for efficient biosensor applications. Our new approach used microfluidic devices to facilitate the single throughput, in-situ process to immobilize enzyme in a nanoporous scaffold via flow induced gelation, under ambient conditions. The nanoporous gel serves as a favorable host matrix for the immobilization of horseradish peroxidase (HRP) and ferrocene methanol (FcMeOH). The biosensor designed by the nanoporous scaffold demonstrated a highly linear amperometric response over the 0.1-1.5 mM range of H2O2 examined, with high sensitivity of 2.5 μM, high stability and selectivity, and good precision (RSD = 2.4%). This flow induced immobilziation technique opens up new pathways for designing simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site testing of a variety of biomolecules.
Original language | English (US) |
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Title of host publication | 10AIChE - 2010 AIChE Annual Meeting, Conference Proceedings |
State | Published - Dec 1 2010 |
Event | 2010 AIChE Annual Meeting, 10AIChE - Salt Lake City, UT, United States Duration: Nov 7 2010 → Nov 12 2010 |
Other
Other | 2010 AIChE Annual Meeting, 10AIChE |
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Country | United States |
City | Salt Lake City, UT |
Period | 11/7/10 → 11/12/10 |
Keywords
- Electrochemical sensor
- Enzyme
- Flow induced gelation
- Immobilization
- Microfluidics
- Nanoporous scaffolds
ASJC Scopus subject areas
- Chemical Engineering(all)
- Chemistry(all)