Nanoparticle-Based Proximity Ligation Assay for Ultrasensitive, Quantitative Detection of Protein Biomarkers

Hui Chen, Mary Crum, Dimple Chavan, Binh Vu, Katerina Kourentzi, Richard C. Willson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Proximity ligation assay (PLA) achieves extremely low limits of detection but requires the synthesis of antibody-DNA conjugates recognizing multiple target epitopes with appropriate proximity. In this work, we introduce a more generally applicable method by replacing antibody-DNA conjugates with nanoparticles which create ultradetectable PCR templates by capturing biotinylated oligonucleotides and catalyzing ligation. A competitive PCR readout was used to make the assay quantitative. We have demonstrated that NP-PLA can detect and quantitate human chorionic gonadotropin (hCG) levels as low as 2.6 fM (∼0.1 pg/mL), nearly 1000 times more sensitive than the current state of the art ELISA.

Original languageEnglish (US)
Pages (from-to)31845-31849
Number of pages5
JournalACS Applied Materials and Interfaces
Volume10
Issue number38
DOIs
StatePublished - Sep 26 2018

Keywords

  • competitive polymerase chain reaction
  • immunoassay
  • nanoparticle
  • protein detection
  • proximity ligation assay

ASJC Scopus subject areas

  • Materials Science(all)

Fingerprint Dive into the research topics of 'Nanoparticle-Based Proximity Ligation Assay for Ultrasensitive, Quantitative Detection of Protein Biomarkers'. Together they form a unique fingerprint.

Cite this