@article{86498a0396ca49a5b04d6c3dcac763b4,
title = "Nanoparticle-Based Proximity Ligation Assay for Ultrasensitive, Quantitative Detection of Protein Biomarkers",
abstract = "Proximity ligation assay (PLA) achieves extremely low limits of detection but requires the synthesis of antibody-DNA conjugates recognizing multiple target epitopes with appropriate proximity. In this work, we introduce a more generally applicable method by replacing antibody-DNA conjugates with nanoparticles which create ultradetectable PCR templates by capturing biotinylated oligonucleotides and catalyzing ligation. A competitive PCR readout was used to make the assay quantitative. We have demonstrated that NP-PLA can detect and quantitate human chorionic gonadotropin (hCG) levels as low as 2.6 fM (∼0.1 pg/mL), nearly 1000 times more sensitive than the current state of the art ELISA.",
keywords = "competitive polymerase chain reaction, immunoassay, nanoparticle, protein detection, proximity ligation assay",
author = "Hui Chen and Mary Crum and Dimple Chavan and Binh Vu and Katerina Kourentzi and Willson, {Richard C.}",
note = "Funding Information: *E-mail: willson@uh.edu. ORCID Richard C. Willson: 0000-0002-0900-6148 Funding We gratefully acknowledge support from National Institute of Allergy and Infectious Diseases, National Institutes of Health, http://www.niaid.nih.gov (Grant 1R21AI111120-01A1);National Science Foundation, www.nsf.gov (Grant CBET-1511789); and Cancer Prevention & Research Institute of Texas, www.cprit.state.tx.us (Grant RP150343). Notes The authors declare the following competing financial interest(s): Several of the authors of this manuscript are named inventors on pending IP which overlaps the topics of the manuscript. Publisher Copyright: {\textcopyright} 2018 American Chemical Society. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2018",
month = sep,
day = "26",
doi = "10.1021/acsami.8b01377",
language = "English (US)",
volume = "10",
pages = "31845--31849",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "38",
}