Nanometric protein arrays on protein-resistant monolayers on silicon surfaces

Jianhua Gu, Chi Ming Yam, Sha Li, Chengzhi Cai

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

We present a novel approach for preparation of nanometric protein arrays, based on binding of avidin molecules to nanotemplates generated by conductive AFM lithography on robust oligo(ethylene glycol)-terminated monolayers on silicon (111) surfaces that are protein-resistant. We showed that only biotinated-BSA but not the native BSA bind to the avidin arrays and that the resulting arrays of biotinated BSA could bind avidin to form protein dots with a feature size of ∼30 nm. This result demonstrates that the avidin array may serve as templates for preparation of nanoarrays of a wide variety of biotin-tagged proteins for studying their interactions with other protein molecules at nanoscale.

Original languageEnglish (US)
Pages (from-to)8098-8099
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number26
DOIs
StatePublished - Jul 7 2004
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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