@inproceedings{62c709b414534bbb976c6a1a0164b0dd,
title = "Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation",
abstract = "Antibodies (Ab) are patterned at nanoscale precision for the precise immobilization and stimulation of immune cells. We demonstrate that the antigen bovine serum albumin (BSA) can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in aqueous solution. Anti-BSA Ab are bound specifically to BSA to create a pattern of oriented Ab that provides a surface for eosinophil immobilization and degranulation. The patterns ranged from 0.36 μm2 to 4,489 μm2, appropriate dimensions for the 10-14 μm diameter eosinophil cells. This method provides a new technique for immobilizing cells onto nano and micrometer scale patterns for analyzing cellular biochemical cascade events such as degranulation and studying cellular morphological changes in response to defined nanoscale antigenic stimulus.",
keywords = "Immunology, biomaterials, nanofabrication, surface modification",
author = "Orth, {R. N.} and L. Kwan and J. Kameoka and M. Lindau and Craighead, {H. G.}",
note = "Funding Information: We acknowledge the support of DARPA, NSF support through the Nanobiotechnology Center, and the resources of the Cornell Nanofabrication Facility.We would like to thank Dr. Ismail Hafez for his assistance preparing lipids and guidance on configuring a lipid extrusion system. We would also like to thank Mathieu Foquet for his designing the microscope set-up for this experiment and guidance with imaging. The views expressed in this article are those of the authors and do not reflect the Official Policy or position of the United States Air Force, Department of Defense, or the U.S. Government. Publisher Copyright: {\textcopyright} 2002 IEEE.; 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology ; Conference date: 02-05-2002 Through 04-05-2002",
year = "2002",
doi = "10.1109/MMB.2002.1002276",
language = "English (US)",
series = "2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "115--119",
editor = "David Beebe and Andre Dittmar",
booktitle = "2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings",
address = "United States",
}