Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation

R. N. Orth; L. Kwan; J. Kameoka; M. Lindau; Harold G. Craighead

doi:10.1109/MMB.2002.1002276

Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation

R. N. Orth, L. Kwan, J. Kameoka, M. Lindau, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Antibodies (Ab) are patterned at nanoscale precision for the precise immobilization and stimulation of immune cells. We demonstrate that the antigen bovine serum albumin (BSA) can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in aqueous solution. Anti-BSA Ab are bound specifically to BSA to create a pattern of oriented Ab that provides a surface for eosinophil immobilization and degranulation. The patterns ranged from 0.36 μm² to 4,489 μm², appropriate dimensions for the 10-14 μm diameter eosinophil cells. This method provides a new technique for immobilizing cells onto nano and micrometer scale patterns for analyzing cellular biochemical cascade events such as degranulation and studying cellular morphological changes in response to defined nanoscale antigenic stimulus.

Original language	English (US)
Title of host publication	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	115-119
Number of pages	5
ISBN (Print)	0780374800, 9780780374805
DOIs	https://doi.org/10.1109/MMB.2002.1002276
State	Published - 2002
Event	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States Duration: May 2 2002 → May 4 2002

Other

Other	2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
Country	United States
City	Madison
Period	5/2/02 → 5/4/02

Keywords

biomaterials
Immunology
nanofabrication
surface modification

ASJC Scopus subject areas

Biotechnology
Microbiology (medical)

Access to Document

10.1109/MMB.2002.1002276

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Orth, R. N., Kwan, L., Kameoka, J., Lindau, M., & Craighead, H. G. (2002). Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation. In 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 115-119). [1002276] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002276

Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation. / Orth, R. N.; Kwan, L.; Kameoka, J.; Lindau, M.; Craighead, Harold G.

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2002. p. 115-119 1002276.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Orth, RN, Kwan, L, Kameoka, J, Lindau, M & Craighead, HG 2002, Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation. in 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 1002276, Institute of Electrical and Electronics Engineers Inc., pp. 115-119, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 5/2/02. https://doi.org/10.1109/MMB.2002.1002276

Orth RN, Kwan L, Kameoka J, Lindau M, Craighead HG. Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation. In 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2002. p. 115-119. 1002276 https://doi.org/10.1109/MMB.2002.1002276

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abstract = "Antibodies (Ab) are patterned at nanoscale precision for the precise immobilization and stimulation of immune cells. We demonstrate that the antigen bovine serum albumin (BSA) can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in aqueous solution. Anti-BSA Ab are bound specifically to BSA to create a pattern of oriented Ab that provides a surface for eosinophil immobilization and degranulation. The patterns ranged from 0.36 μm2 to 4,489 μm2, appropriate dimensions for the 10-14 μm diameter eosinophil cells. This method provides a new technique for immobilizing cells onto nano and micrometer scale patterns for analyzing cellular biochemical cascade events such as degranulation and studying cellular morphological changes in response to defined nanoscale antigenic stimulus.",

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