Nanometer-scale antibody patterning for directed eosinophil cell immobilization and stimulation

R. N. Orth, L. Kwan, J. Kameoka, M. Lindau, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Antibodies (Ab) are patterned at nanoscale precision for the precise immobilization and stimulation of immune cells. We demonstrate that the antigen bovine serum albumin (BSA) can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in aqueous solution. Anti-BSA Ab are bound specifically to BSA to create a pattern of oriented Ab that provides a surface for eosinophil immobilization and degranulation. The patterns ranged from 0.36 μm2 to 4,489 μm2, appropriate dimensions for the 10-14 μm diameter eosinophil cells. This method provides a new technique for immobilizing cells onto nano and micrometer scale patterns for analyzing cellular biochemical cascade events such as degranulation and studying cellular morphological changes in response to defined nanoscale antigenic stimulus.

Original languageEnglish (US)
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages115-119
Number of pages5
ISBN (Print)0780374800, 9780780374805
DOIs
StatePublished - 2002
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: May 2 2002May 4 2002

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period5/2/025/4/02

Keywords

  • biomaterials
  • Immunology
  • nanofabrication
  • surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology (medical)

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