Nanofluidic structures for single biomolecule fluorescent detection

J. T. Mannion; H. G. Craighead

doi:10.1002/bip.20629

Nanofluidic structures for single biomolecule fluorescent detection

J. T. Mannion, H. G. Craighead

Houston Methodist

Research output: Contribution to journal › Review article › peer-review

67 Scopus citations

Abstract

Fluid-filled nanofabricated cavities can be used to increase the spatial resolution of single molecule confocal microscopy based techniques by creating smaller and more uniformly illuminated probe volumes. Such structures may also be used to temporarily stretch single macromolecules, permitting the resolution of molecular details that would otherwise be beyond the capabilities of a diffraction limited system.

Original language	English (US)
Pages (from-to)	131-143
Number of pages	13
Journal	Biopolymers
Volume	85
Issue number	2
DOIs	https://doi.org/10.1002/bip.20629
State	Published - Feb 2007

Keywords

DNA
Nanochannel
Nanofluidic
Single molecule
Zero-mode waveguide

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Biochemistry
Biophysics

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10.1002/bip.20629

Cite this

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title = "Nanofluidic structures for single biomolecule fluorescent detection",

abstract = "Fluid-filled nanofabricated cavities can be used to increase the spatial resolution of single molecule confocal microscopy based techniques by creating smaller and more uniformly illuminated probe volumes. Such structures may also be used to temporarily stretch single macromolecules, permitting the resolution of molecular details that would otherwise be beyond the capabilities of a diffraction limited system.",

keywords = "DNA, Nanochannel, Nanofluidic, Single molecule, Zero-mode waveguide",

author = "Mannion, {J. T.} and Craighead, {H. G.}",

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AB - Fluid-filled nanofabricated cavities can be used to increase the spatial resolution of single molecule confocal microscopy based techniques by creating smaller and more uniformly illuminated probe volumes. Such structures may also be used to temporarily stretch single macromolecules, permitting the resolution of molecular details that would otherwise be beyond the capabilities of a diffraction limited system.

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KW - Nanochannel

KW - Nanofluidic

KW - Single molecule

KW - Zero-mode waveguide

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Nanofluidic structures for single biomolecule fluorescent detection

Abstract

Keywords

ASJC Scopus subject areas

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