Nanofluidic structures for single biomolecule fluorescent detection

J. T. Mannion, H. G. Craighead

Research output: Contribution to journalReview article

63 Scopus citations

Abstract

Fluid-filled nanofabricated cavities can be used to increase the spatial resolution of single molecule confocal microscopy based techniques by creating smaller and more uniformly illuminated probe volumes. Such structures may also be used to temporarily stretch single macromolecules, permitting the resolution of molecular details that would otherwise be beyond the capabilities of a diffraction limited system.

Original languageEnglish (US)
Pages (from-to)131-143
Number of pages13
JournalBiopolymers
Volume85
Issue number2
DOIs
StatePublished - Feb 2007

Keywords

  • DNA
  • Nanochannel
  • Nanofluidic
  • Single molecule
  • Zero-mode waveguide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

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