TY - JOUR
T1 - Nanocomposite Hydrogels as Platform for Cells Growth, Proliferation, and Chemotaxis
AU - Fiorini, Federica
AU - Prasetyanto, Eko Adi
AU - Taraballi, Francesca
AU - Pandolfi, Laura
AU - Monroy, Francisco
AU - López-Montero, Iván
AU - Tasciotti, Ennio
AU - De Cola, Luisa
N1 - © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/9
Y1 - 2016/9
N2 - The challenge of mimicking the extracellular matrix with artificial scaffolds that are able to reduce immunoresponse is still unmet. Recent findings have shown that mesenchymal stem cells (MSC) infiltrating into the implanted scaffold have effects on the implant integration by improving the healing process. Toward this aim, a novel polyamidoamine-based nanocomposite hydrogel is synthesized, cross-linked with porous nanomaterials (i.e., mesoporous silica nanoparticles), able to release chemokine proteins. A comprehensive viscoelasticity study confirms that the hydrogel provides optimal structural support for MSC infiltration and proliferation. The efficiency of this hydrogel, containing the chemoattractant stromal cell-derived factor 1α (SDF-1α), in promoting MSC migration in vitro is demonstrated. Finally, subcutaneous implantation of SDF-1α-releasing hydrogels in mice results in a modulation of the inflammatory reaction. Overall, the proposed SDF-1α-nanocomposite hydrogel proves to have potential for applications in tissue engineering.
AB - The challenge of mimicking the extracellular matrix with artificial scaffolds that are able to reduce immunoresponse is still unmet. Recent findings have shown that mesenchymal stem cells (MSC) infiltrating into the implanted scaffold have effects on the implant integration by improving the healing process. Toward this aim, a novel polyamidoamine-based nanocomposite hydrogel is synthesized, cross-linked with porous nanomaterials (i.e., mesoporous silica nanoparticles), able to release chemokine proteins. A comprehensive viscoelasticity study confirms that the hydrogel provides optimal structural support for MSC infiltration and proliferation. The efficiency of this hydrogel, containing the chemoattractant stromal cell-derived factor 1α (SDF-1α), in promoting MSC migration in vitro is demonstrated. Finally, subcutaneous implantation of SDF-1α-releasing hydrogels in mice results in a modulation of the inflammatory reaction. Overall, the proposed SDF-1α-nanocomposite hydrogel proves to have potential for applications in tissue engineering.
KW - chemotaxis
KW - hydrogel
KW - in vivo implant
KW - mesoporous particles
KW - nanocomposites
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U2 - 10.1002/smll.201601017
DO - 10.1002/smll.201601017
M3 - Article
C2 - 27364463
AN - SCOPUS:84978064521
VL - 12
SP - 4881
EP - 4893
JO - Small
JF - Small
SN - 1613-6810
IS - 35
ER -