TY - JOUR
T1 - Myeloma bone marrow plasma cells
T2 - Evidence for their capacity as antigen-presenting cells
AU - Yi, Qing
AU - Dabadghao, Sunil
AU - Österborg, Anders
AU - Bergenbrant, Susanne
AU - Holm, Göran
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1997/9/1
Y1 - 1997/9/1
N2 - Myeloma plasma calls constitute 10% to 90% of the total bone marrow cell count in patients with multiple myeloma (MM). These cells express a variety of cell surface markers, such as HLA-ABC and HLA-DR, and surface antigens that are necessary for professional antigen-presenting cells, including adhesion and costimulatory molecules. In this study, we examined the expression of major histocompatability complex (MHC) and costimulatory molecules on CD38((bright,++)) plasma cells in bone marrow aspirates from eight MM patients. Small percentages of plasma cells expressed weak but detectable levels of HLA-DR, HLA-DQ, CD40, CD80, and CD86, which could be upregulated by interferon-γ (IFN-γ) and tumor necrosis factor-α. CD38++ plasma cell and CD38((dlm,+)) cells were sorted from freshly isolated bone marrow mononuclear cells and tested for their capacity to act as antigen- presenting cells. Indeed, both CD38++ plasma cells and CD38+ cells were able to stimulate allogeneic T cells and present the soluble antigens purified protein derivative and tetanus toxoid to autologous T cells. Recognition of the antigens led to T-cell proliferation and secretion of IFN- γ and was MHC class-I and -II restricted. Antigen processing and presentation by CD38++ and CD38+ cells were abolished by treatment of the cells with chloroquine. Hence, our study provides for the first time evidence that myeloma plasma cells may act as antigen-presenting cells. Further studies are warranted to examine in detail the molecules required for inducing T-cell stimulation.
AB - Myeloma plasma calls constitute 10% to 90% of the total bone marrow cell count in patients with multiple myeloma (MM). These cells express a variety of cell surface markers, such as HLA-ABC and HLA-DR, and surface antigens that are necessary for professional antigen-presenting cells, including adhesion and costimulatory molecules. In this study, we examined the expression of major histocompatability complex (MHC) and costimulatory molecules on CD38((bright,++)) plasma cells in bone marrow aspirates from eight MM patients. Small percentages of plasma cells expressed weak but detectable levels of HLA-DR, HLA-DQ, CD40, CD80, and CD86, which could be upregulated by interferon-γ (IFN-γ) and tumor necrosis factor-α. CD38++ plasma cell and CD38((dlm,+)) cells were sorted from freshly isolated bone marrow mononuclear cells and tested for their capacity to act as antigen- presenting cells. Indeed, both CD38++ plasma cells and CD38+ cells were able to stimulate allogeneic T cells and present the soluble antigens purified protein derivative and tetanus toxoid to autologous T cells. Recognition of the antigens led to T-cell proliferation and secretion of IFN- γ and was MHC class-I and -II restricted. Antigen processing and presentation by CD38++ and CD38+ cells were abolished by treatment of the cells with chloroquine. Hence, our study provides for the first time evidence that myeloma plasma cells may act as antigen-presenting cells. Further studies are warranted to examine in detail the molecules required for inducing T-cell stimulation.
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U2 - 10.1182/blood.v90.5.1960
DO - 10.1182/blood.v90.5.1960
M3 - Article
C2 - 9292530
AN - SCOPUS:0030884240
SN - 0006-4971
VL - 90
SP - 1960
EP - 1967
JO - Blood
JF - Blood
IS - 5
ER -