TY - JOUR
T1 - Mycobacterium avium serovars 2 and 8 infections elicit unique activation of the host macrophage immune responses
AU - Cebula, B. R.
AU - Rocco, J. M.
AU - Maslow, J. N.
AU - Irani, V. R.
N1 - Funding Information:
Acknowledgments The authors of this manuscript report no personal or professional relationships which could pose a conflict of interest. This research was supported by a Pennsylvania State System of Higher Education (PASSHE) Faculty Professional Development Council (FPDC) award to V.R. Irani (2007); the 2010 Cynthia Sushak Undergraduate Biology Fund for Excellence Award to B.R. Cebula and J.M. Rocco; and an Indiana University of Pennsylvania (IUP) School of Graduate Studies and Research (SGSR) Undergraduate Award to B.R. Cebula (2010) and J.M. Rocco (2009).
PY - 2012/12
Y1 - 2012/12
N2 - Mycobacterium avium is an opportunistic pathogen whose pathogenesis is attributed to its serovar-specific glycopeptidolipid (ssGPL), which varies among its 31 serovars. To determine if the presence and type of ssGPLs contribute to M. avium pathogenesis, we infected murine macrophages (mωs) with two M. avium wild type (wt) serovars (2 and 8) and their serovar-null strains. We examined the influence of ssGPL (presence and type) on cytokine production in non-activated (?IFN-γ) and activated (+IFN- γ) mωs, and the bacterial intra-mω survival over a 6-day infection process. Serovar-2 infections activated TNF-α production that increased over the 6 day period and was capable of controlling the intra-mω serovar-2 null strain. In contrast, the serovar-8 infection stimulated a strong proinflammatory response, but was incapable of removing the invading pathogen, maybe through IL-10 production. It was clear that the intracellular growth of serovar-null in contrast to the wt M. avium strains was easily controlled. Based on our findings and the undisputed fact that M. avium ssGPL is key to its pathogenesis, we conclude that it is not appropriate to dissect the pathogenesis of one M. avium serovar and apply those findings to other serovars.
AB - Mycobacterium avium is an opportunistic pathogen whose pathogenesis is attributed to its serovar-specific glycopeptidolipid (ssGPL), which varies among its 31 serovars. To determine if the presence and type of ssGPLs contribute to M. avium pathogenesis, we infected murine macrophages (mωs) with two M. avium wild type (wt) serovars (2 and 8) and their serovar-null strains. We examined the influence of ssGPL (presence and type) on cytokine production in non-activated (?IFN-γ) and activated (+IFN- γ) mωs, and the bacterial intra-mω survival over a 6-day infection process. Serovar-2 infections activated TNF-α production that increased over the 6 day period and was capable of controlling the intra-mω serovar-2 null strain. In contrast, the serovar-8 infection stimulated a strong proinflammatory response, but was incapable of removing the invading pathogen, maybe through IL-10 production. It was clear that the intracellular growth of serovar-null in contrast to the wt M. avium strains was easily controlled. Based on our findings and the undisputed fact that M. avium ssGPL is key to its pathogenesis, we conclude that it is not appropriate to dissect the pathogenesis of one M. avium serovar and apply those findings to other serovars.
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U2 - 10.1007/s10096-012-1709-4
DO - 10.1007/s10096-012-1709-4
M3 - Article
C2 - 22991047
AN - SCOPUS:84872173684
VL - 31
SP - 3407
EP - 3412
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
SN - 0934-9723
IS - 12
ER -