Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori

James Versalovic; Dee Shortridge; Kirsten Kibler; Mamie V. Griffy; Jill Beyer; Robert K. Flamm; S. Ken Tanaka; David Y. Graham; Mae F. Go

doi:10.1128/aac.40.2.477

Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori

James Versalovic, Dee Shortridge, Kirsten Kibler, Mamie V. Griffy, Jill Beyer, Robert K. Flamm, S. Ken Tanaka, David Y. Graham, Mae F. Go

Research output: Contribution to journal › Article › peer-review

433 Scopus citations

Abstract

Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A→G transition mutation within a conserved loop of 23S rRNA. A→G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.

Original language	English (US)
Pages (from-to)	477-480
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	40
Issue number	2
DOIs	https://doi.org/10.1128/aac.40.2.477
State	Published - Feb 1996

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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title = "Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori",

abstract = "Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A→G transition mutation within a conserved loop of 23S rRNA. A→G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.",

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AU - Versalovic, James

AU - Shortridge, Dee

AU - Kibler, Kirsten

AU - Griffy, Mamie V.

AU - Beyer, Jill

AU - Flamm, Robert K.

AU - Tanaka, S. Ken

AU - Graham, David Y.

AU - Go, Mae F.

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N2 - Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A→G transition mutation within a conserved loop of 23S rRNA. A→G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.

AB - Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A→G transition mutation within a conserved loop of 23S rRNA. A→G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.

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Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori

Abstract

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