Mutagenic potential of binary and complex mixtures using different enzyme induction systems

K. V. Markiewicz, L. E. Howie, S. H. Safe, K. C. Donnelly

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


A series of experiments was conducted to investigate the mutagenic potential of binary and complex mixtures in the presence and absence of inducible liver enzyme systems prepared with several different chemical inducers. Liver homogenate (S9, or 9000 g supernatant) fractions were obtained from Sprague-Dawley rats induced with either Aroclor 1254 (AR), phenobarbital (PB), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or corn oil (UI). The mutagenic potential of test samples was m easured with Salmonella typhimurium strain TA98 using each of the various S9 fractions. Test samples included benzo[a]pyrene (BaP), pentachlorophenol (PCP), a binary mixture of BaP and PCP, two five-component mixtures, a methylene chloride extract of wood preserving waste-amended soil, and a methanol extract of coal gasification waste. At a dose of 25 mu g/plate, BaP produced 55, 83, 217, and 161 net revertants per plate with UI-, PB-, AR-, and TCDD-induced S9, respectively. The complex mixture extracted from the wood preserving waste-amended soil induced approximately equal responses with all four S9 mixes. At a dose of 250 mu g/plate, the methanol extract of a coal gasification waste produced 56 net revertants using the uninduced S9; however, when AR-and TCDD-induced S9 was used, 129 and 67 net revertants were observed, respectively. These data demonstrate the relative im portance of the various induced cytochrome P-450 isozymes for the metabolism of mutagenic chemicals and complex mixtures.

Original languageEnglish (US)
Pages (from-to)443-451
Number of pages9
JournalJournal of Toxicology and Environmental Health
Issue number5
StatePublished - Mar 1996

ASJC Scopus subject areas

  • Toxicology
  • Pollution


Dive into the research topics of 'Mutagenic potential of binary and complex mixtures using different enzyme induction systems'. Together they form a unique fingerprint.

Cite this