TY - JOUR
T1 - Mutagenic and cytotoxic action of heated pork meat extracts in human diploid fibroblasts
AU - Kolman, Ada
AU - Kleman, Marika
AU - Bohušová, Tatiana
AU - Gustafsson, Jan Ake
AU - Övervik, Eva
PY - 1992/3
Y1 - 1992/3
N2 - Extracts from lean pork heated at 200°C have a strong mutagenic activity in the Ames Salmonella assay (strain TA98 + S9). The formation of mutagenicity is highly temperature dependent, thus an extract heated at 100°C is not mutagenic in this system. This paper shows that the 200°C extract also causes mutations at the hprt locus in normal human fibroblasts, as demonstrated by a dose-dependent increase of 6-thioguanine resistant mutants. The mutation frequencies were increased 6 and 13 times, respectively, for extract concentrations corresponding to 100 and 200 mg meat/ml medium. Heterocyclic amines, previously shown to be present in the 200°C extract are conceivably responsible for at least part of the observed mutagenicity. The extracts prepared at 100°C had no significant effect on the mutant frequency in human fibroblasts. A pronounced, dose-dependent, decrease in cell survival was observed with both the 100° and the 200°C extracts. The nature of the cytotoxic components is not clear and might be different in the two extracts.
AB - Extracts from lean pork heated at 200°C have a strong mutagenic activity in the Ames Salmonella assay (strain TA98 + S9). The formation of mutagenicity is highly temperature dependent, thus an extract heated at 100°C is not mutagenic in this system. This paper shows that the 200°C extract also causes mutations at the hprt locus in normal human fibroblasts, as demonstrated by a dose-dependent increase of 6-thioguanine resistant mutants. The mutation frequencies were increased 6 and 13 times, respectively, for extract concentrations corresponding to 100 and 200 mg meat/ml medium. Heterocyclic amines, previously shown to be present in the 200°C extract are conceivably responsible for at least part of the observed mutagenicity. The extracts prepared at 100°C had no significant effect on the mutant frequency in human fibroblasts. A pronounced, dose-dependent, decrease in cell survival was observed with both the 100° and the 200°C extracts. The nature of the cytotoxic components is not clear and might be different in the two extracts.
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U2 - 10.1093/mutage/7.2.141
DO - 10.1093/mutage/7.2.141
M3 - Article
C2 - 1579070
AN - SCOPUS:0026773534
VL - 7
SP - 141
EP - 144
JO - Mutagenesis
JF - Mutagenesis
SN - 0267-8357
IS - 2
ER -