TY - JOUR
T1 - Mutagenic and cytotoxic action of heated pork meat extracts in human diploid fibroblasts
AU - Kolman, Ada
AU - Kleman, Marika
AU - Bohušová, Tatiana
AU - Gustafsson, Jan Ake
AU - Övervik, Eva
N1 - Funding Information:
The authors are grateful to Professor Bo Lambert, Department of Clinical Genetics, Karolinska Institute, for fruitful discussions and recommendations. The work was financially supported by the Swedish Cancer Society and the Swedish Council for Forestry and Agricultural Research. T.B. is grateful for a grant from the European Science Foundation Program of Research Fellowships in Toxicology (Strasbourg, France).
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1992/3
Y1 - 1992/3
N2 - Extracts from lean pork heated at 200°C have a strong mutagenic activity in the Ames Salmonella assay (strain TA98 + S9). The formation of mutagenicity is highly temperature dependent, thus an extract heated at 100°C is not mutagenic in this system. This paper shows that the 200°C extract also causes mutations at the hprt locus in normal human fibroblasts, as demonstrated by a dose-dependent increase of 6-thioguanine resistant mutants. The mutation frequencies were increased 6 and 13 times, respectively, for extract concentrations corresponding to 100 and 200 mg meat/ml medium. Heterocyclic amines, previously shown to be present in the 200°C extract are conceivably responsible for at least part of the observed mutagenicity. The extracts prepared at 100°C had no significant effect on the mutant frequency in human fibroblasts. A pronounced, dose-dependent, decrease in cell survival was observed with both the 100° and the 200°C extracts. The nature of the cytotoxic components is not clear and might be different in the two extracts.
AB - Extracts from lean pork heated at 200°C have a strong mutagenic activity in the Ames Salmonella assay (strain TA98 + S9). The formation of mutagenicity is highly temperature dependent, thus an extract heated at 100°C is not mutagenic in this system. This paper shows that the 200°C extract also causes mutations at the hprt locus in normal human fibroblasts, as demonstrated by a dose-dependent increase of 6-thioguanine resistant mutants. The mutation frequencies were increased 6 and 13 times, respectively, for extract concentrations corresponding to 100 and 200 mg meat/ml medium. Heterocyclic amines, previously shown to be present in the 200°C extract are conceivably responsible for at least part of the observed mutagenicity. The extracts prepared at 100°C had no significant effect on the mutant frequency in human fibroblasts. A pronounced, dose-dependent, decrease in cell survival was observed with both the 100° and the 200°C extracts. The nature of the cytotoxic components is not clear and might be different in the two extracts.
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U2 - 10.1093/mutage/7.2.141
DO - 10.1093/mutage/7.2.141
M3 - Article
C2 - 1579070
AN - SCOPUS:0026773534
VL - 7
SP - 141
EP - 144
JO - Mutagenesis
JF - Mutagenesis
SN - 0267-8357
IS - 2
ER -