In the United States, peripheral arterial disease (PAD) affects about 10 million individuals, and is also prevalent worldwide. Medical therapies forsymptomatic relief are limited. Surgical or endovascular interventions are useful for some individuals, but long-term results are often disappointing.As a result, there is a need for developing new therapies to treat PAD. The murine hindlimb ischemia preparation is a model of PAD, and is usefulfor testing new therapies. When compared to other models of tissue ischemia such as coronary or cerebral artery ligation, femoral artery ligationprovides for a simpler model of ischemic tissue. Other advantages of this model are the ease of access to the femoral artery and low mortality rate.In this video, we demonstrate the methodology for the murine model of unilateral hindimb ischemia. The specific materials and procedures forcreating and evaluating the model will be described, including the assessment of limb perfusion by laser Doppler imaging. This protocol can also beutilized for the transplantation and non-invasive tracking of cells, which is demonstrated by Huang et al.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)