Multivariate analysis approach to the plasma protein profile of patients with advanced colorectal cancer

Eugenio Ragazzi, Salvatore Pucciarelli, Roberta Seraglia, Laura Molin, Marco Agostini, Mario Lise, Pietro Traldi, Donato Nitti

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The aim of the present study was to identify the pattern of plasma protein species of interest as markers of colorectal cancer (CRC). Using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), the plasma protein profile was determined in nine stage IV CRC patients (study group) and nine clean-colon healthy subjects (control group). Multivariate analysis methods were employed to identify distinctive disease patterns at protein spectrum. In the study and control groups, cluster analysis (CA) on the complete MALDI-MS spectra plasma protein profile showed a distinction between CRC patients and healthy subjects, thus allowing the identification of the most discriminating ionic species. Principal component analysis (PCA) and linear discriminant analysis (LDA) yielded similar grouping results. LDA with leave-one-out cross validation achieved a correct classification rate of 89% in both the patients and the healthy subjects.

Original languageEnglish (US)
Pages (from-to)1546-1553
Number of pages8
JournalJournal of Mass Spectrometry
Volume41
Issue number12
DOIs
StatePublished - Dec 1 2006

Keywords

  • Colorectal cancer
  • LDA
  • LMW plasma protein profile
  • MALDI/MS
  • PCA

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

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