Multiscale benchmarking of drug delivery vectors

Huw D. Summers, Matthew J. Ware, Ravish Majithia, Kenith E. Meissner, Biana Godin, Paul Rees

Research output: Contribution to journalArticle

Abstract

Cross-system comparisons of drug delivery vectors are essential to ensure optimal design. An in-vitro experimental protocol is presented that separates the role of the delivery vector from that of its cargo in determining the cell response, thus allowing quantitative comparison of different systems. The technique is validated through benchmarking of the dose-response of human fibroblast cells exposed to the cationic molecule, polyethylene imine (PEI); delivered as a free molecule and as a cargo on the surface of CdSe nanoparticles and Silica microparticles. The exposure metrics are converted to a delivered dose with the transport properties of the different scale systems characterized by a delivery time, τ. The benchmarking highlights an agglomeration of the free PEI molecules into micron sized clusters and identifies the metric determining cell death as the total number of PEI molecules presented to cells, determined by the delivery vector dose and the surface density of the cargo.

Original languageEnglish (US)
Pages (from-to)1843-1851
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume12
Issue number7
DOIs
StatePublished - Oct 1 2016

Keywords

  • Dose-Response assays
  • Drug delivery
  • Nanomedicine
  • Nanoparticles
  • Nanotoxicology

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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