Multiplexed immunoassay biosensor for the detection of serum biomarkers - β-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots

Dan Liu; Feng Wu; Changhua Zhou; Huaibing Shen; Hang Yuan; Zuliang Du; Lan Ma; Lin Song Li

doi:10.1016/j.snb.2013.05.094

Multiplexed immunoassay biosensor for the detection of serum biomarkers - β-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots

Dan Liu, Feng Wu, Changhua Zhou, Huaibing Shen, Hang Yuan, Zuliang Du, Lan Ma, Lin Song Li

Houston Methodist

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A multiplexed immunoassay biosensor for detecting serum biomarkers was developed by using photoluminescent (PL) aqueous CdSe/ZnS quantum dots (QDs) with high efficiency and stability as fluorescence labels and carboxyl functionalized microporous nylon membrane as a matrix. Two main biomarkers of Down Syndrome (DS), β-human chorionic gonadotrophin (β-HCG) and α-fetal protein (AFP), were selected for detection in this biosensor system. For specific detection of DS, antibody-immobilized microporous nylon membrane first captured the targeted antigens from the physiological samples and formed immunocomplexes (antibody-antigen) on the membrane surface. Then the immunocomplexes continued to react with QDs conjugated antibodies (labeled antibodies), and formed triplex "sandwich" structure, which was confirmed by AFM and confocal microscopy. Quantitative detection of β-HCG and AFP was also realized, the detectable level of this new biosensor was as low as 1 × 10^-6 IU/L for β-HCG and 1 ng/mL for AFP in a relatively short assay time without any enrichment.

Original language	English (US)
Pages (from-to)	235-243
Number of pages	9
Journal	Sensors and Actuators, B: Chemical
Volume	186
DOIs	https://doi.org/10.1016/j.snb.2013.05.094
State	Published - 2013

Keywords

β-HCG
AFP
DS
Immunomembrane
Multiplexed biosensor
PL
QDs

ASJC Scopus subject areas

Instrumentation
Materials Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Electrical and Electronic Engineering

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10.1016/j.snb.2013.05.094

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title = "Multiplexed immunoassay biosensor for the detection of serum biomarkers - β-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots",

abstract = "A multiplexed immunoassay biosensor for detecting serum biomarkers was developed by using photoluminescent (PL) aqueous CdSe/ZnS quantum dots (QDs) with high efficiency and stability as fluorescence labels and carboxyl functionalized microporous nylon membrane as a matrix. Two main biomarkers of Down Syndrome (DS), β-human chorionic gonadotrophin (β-HCG) and α-fetal protein (AFP), were selected for detection in this biosensor system. For specific detection of DS, antibody-immobilized microporous nylon membrane first captured the targeted antigens from the physiological samples and formed immunocomplexes (antibody-antigen) on the membrane surface. Then the immunocomplexes continued to react with QDs conjugated antibodies (labeled antibodies), and formed triplex {"}sandwich{"} structure, which was confirmed by AFM and confocal microscopy. Quantitative detection of β-HCG and AFP was also realized, the detectable level of this new biosensor was as low as 1 × 10-6 IU/L for β-HCG and 1 ng/mL for AFP in a relatively short assay time without any enrichment.",

keywords = "β-HCG, AFP, DS, Immunomembrane, Multiplexed biosensor, PL, QDs",

author = "Dan Liu and Feng Wu and Changhua Zhou and Huaibing Shen and Hang Yuan and Zuliang Du and Lan Ma and Li, {Lin Song}",

note = "Funding Information: This work was supported by the Program for New Century Excellent Talents in University of Chinese Ministry of Education ( NCET-09-0119 ) and National Natural Science Foundation of China ( 21071041 ), and Changjiang Scholars and Innovative Research Team in University (No. PCS IRT1126 ). ",

year = "2013",

doi = "10.1016/j.snb.2013.05.094",

language = "English (US)",

volume = "186",

pages = "235--243",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Multiplexed immunoassay biosensor for the detection of serum biomarkers - β-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots

AU - Liu, Dan

AU - Wu, Feng

AU - Zhou, Changhua

AU - Shen, Huaibing

AU - Yuan, Hang

AU - Du, Zuliang

AU - Ma, Lan

AU - Li, Lin Song

N1 - Funding Information: This work was supported by the Program for New Century Excellent Talents in University of Chinese Ministry of Education ( NCET-09-0119 ) and National Natural Science Foundation of China ( 21071041 ), and Changjiang Scholars and Innovative Research Team in University (No. PCS IRT1126 ).

PY - 2013

Y1 - 2013

N2 - A multiplexed immunoassay biosensor for detecting serum biomarkers was developed by using photoluminescent (PL) aqueous CdSe/ZnS quantum dots (QDs) with high efficiency and stability as fluorescence labels and carboxyl functionalized microporous nylon membrane as a matrix. Two main biomarkers of Down Syndrome (DS), β-human chorionic gonadotrophin (β-HCG) and α-fetal protein (AFP), were selected for detection in this biosensor system. For specific detection of DS, antibody-immobilized microporous nylon membrane first captured the targeted antigens from the physiological samples and formed immunocomplexes (antibody-antigen) on the membrane surface. Then the immunocomplexes continued to react with QDs conjugated antibodies (labeled antibodies), and formed triplex "sandwich" structure, which was confirmed by AFM and confocal microscopy. Quantitative detection of β-HCG and AFP was also realized, the detectable level of this new biosensor was as low as 1 × 10-6 IU/L for β-HCG and 1 ng/mL for AFP in a relatively short assay time without any enrichment.

AB - A multiplexed immunoassay biosensor for detecting serum biomarkers was developed by using photoluminescent (PL) aqueous CdSe/ZnS quantum dots (QDs) with high efficiency and stability as fluorescence labels and carboxyl functionalized microporous nylon membrane as a matrix. Two main biomarkers of Down Syndrome (DS), β-human chorionic gonadotrophin (β-HCG) and α-fetal protein (AFP), were selected for detection in this biosensor system. For specific detection of DS, antibody-immobilized microporous nylon membrane first captured the targeted antigens from the physiological samples and formed immunocomplexes (antibody-antigen) on the membrane surface. Then the immunocomplexes continued to react with QDs conjugated antibodies (labeled antibodies), and formed triplex "sandwich" structure, which was confirmed by AFM and confocal microscopy. Quantitative detection of β-HCG and AFP was also realized, the detectable level of this new biosensor was as low as 1 × 10-6 IU/L for β-HCG and 1 ng/mL for AFP in a relatively short assay time without any enrichment.

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KW - AFP

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KW - Multiplexed biosensor

KW - PL

KW - QDs

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ER -

Multiplexed immunoassay biosensor for the detection of serum biomarkers - β-HCG and AFP of Down Syndrome based on photoluminescent water-soluble CdSe/ZnS quantum dots

Abstract

Keywords

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