Multiplex in vitro selection of aptamers using nanoporous sol-gel arrays with integrated microheaters

Seung Min Park, Abdullah Ozer, Kylan Szeto, Pangshun Zhu, Raymond Wu, Jiyoung Ahn, Minjoung Jo, Dong Ki Lee, Soyoun Kim, John T. Lis, Moonsoo Jin, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

RNA and DNA aptamers that bind to target molecules with high specificity and affinity have been a focus of diagnostics and therapeutic research. These aptamers are obtained by SELEX (Systematic Evolution of Ligands by EXponential enrichment) often requiring more than 10 successive cycles of selection and amplification, where each cycle normally takes 2 days per cycle of SELEX. Here, we have demonstrated the use of sol-gel arrays of proteins in a microfluidic system for efficient selection of RNA aptamers against multiple target molecules.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages408-411
Number of pages4
ISBN (Print)9780979806421
StatePublished - Jan 1 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Keywords

  • Aptamer
  • Microfluidic
  • SELEX
  • Sol-gel

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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