Multiple specific binding sites for purified glucocorticoid receptors on mammary tumor virus DNA

Farhang Payvar, Gary L. Firestone, Susan R. Ross, Vicki L. Chandler, Örjan Wrange, Jan Carlstedt‐Duke, Jan-Ake Gustafsson, Keith R. Yamamoto

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Glucocorticoid hormones selectively stimulate the rate of transcription of integrated mammary tumor virus (MTV) sequences in infected rat hepatoma cells. Using two independent assays, we find that purified rat liver glucocorticoid receptor protein binds specifically to at least four widely separated regions on pure MTV proviral DNA. One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a fragment of viral DNA that maps 110-449 bp upstream of the promoter for MTV RNA synthesis. Three other binding domains lie downstream of the promoter and within the MTV primary transcription unit. Restriction fragments bearing separate binding domains have been introduced into cultured cells; transformants have been recovered in which the introduced fragments are expressed under glucocorticoid control. Thus, it appears that this assay will be useful for assessing the biological significance of the receptor binding sites detected in vitro.

Original languageEnglish (US)
Pages (from-to)241-247
Number of pages7
JournalJournal of Cellular Biochemistry
Volume19
Issue number3
DOIs
StatePublished - Jan 1 1982

Keywords

  • glucocorticoid receptors
  • mouse mammary tumor virus
  • protein–DNA interactions
  • steroid hormone action
  • transcriptiotial regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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