Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

James Mancuso; Adam M. Larson; Theodore G. Wensel; Peter Saggau

doi:10.1117/1.3139850

Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

James Mancuso, Adam M. Larson, Theodore G. Wensel, Peter Saggau

Academic Institute

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

Original language	English (US)
Article number	034048
Journal	Journal of Biomedical Optics
Volume	14
Issue number	3
DOIs	https://doi.org/10.1117/1.3139850
State	Published - 2009

Keywords

confocal optics
fiber coupled detection
instrumentation
microscopy
multiphoton processes
pulsed laser

ASJC Scopus subject areas

Biomedical Engineering
Biomaterials
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

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10.1117/1.3139850

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title = "Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging",

abstract = "The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.",

keywords = "confocal optics, fiber coupled detection, instrumentation, microscopy, multiphoton processes, pulsed laser",

author = "James Mancuso and Larson, {Adam M.} and Wensel, {Theodore G.} and Peter Saggau",

note = "Funding Information: The presented work was supported by NIH training grant No. EY T32 EY07102 (J.J.M.), NIH Grants No. R01-EY11900 and No. R01-DA015189, and Welch Foundation Grant No. Q0035 (T.G.W.) as well as Grants No. NIH-NIA/RO1 AG027577 and No. NSF/DBI-0455905 (P.S.).",

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AU - Mancuso, James

AU - Larson, Adam M.

AU - Wensel, Theodore G.

AU - Saggau, Peter

N1 - Funding Information: The presented work was supported by NIH training grant No. EY T32 EY07102 (J.J.M.), NIH Grants No. R01-EY11900 and No. R01-DA015189, and Welch Foundation Grant No. Q0035 (T.G.W.) as well as Grants No. NIH-NIA/RO1 AG027577 and No. NSF/DBI-0455905 (P.S.).

PY - 2009

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N2 - The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

AB - The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

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KW - instrumentation

KW - microscopy

KW - multiphoton processes

KW - pulsed laser

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Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

Abstract

Keywords

ASJC Scopus subject areas

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