Multiphoton adaptation of a commercial low-cost confocal microscope for live tissue imaging

James Mancuso, Adam M. Larson, Theodore G. Wensel, Peter Saggau

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.

Original languageEnglish (US)
Article number034048
JournalJournal of Biomedical Optics
Issue number3
StatePublished - 2009


  • confocal optics
  • fiber coupled detection
  • instrumentation
  • microscopy
  • multiphoton processes
  • pulsed laser

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


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