Abstract
The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.
Original language | English (US) |
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Article number | 034048 |
Journal | Journal of Biomedical Optics |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - 2009 |
Keywords
- confocal optics
- fiber coupled detection
- instrumentation
- microscopy
- multiphoton processes
- pulsed laser
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics