Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography

Chunyu Zhang, Kai Zhang, Yanyan Cui, Yuecong Guo, Chuan Wang, Chao Xu, Qingqiang Yao, Yuliang Zhao, Chunying Chen, Yaling Wang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.

Original languageEnglish (US)
Pages (from-to)2129-2133
Number of pages5
JournalAnalytical Chemistry
Issue number4
StatePublished - Jan 31 2023

ASJC Scopus subject areas

  • Analytical Chemistry


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