TY - JOUR
T1 - Multifunctional Nanoprobe for 3D Nanoresolution Imaging of Intact Cell HER2 Protein with Hard X-ray Tomography
AU - Zhang, Chunyu
AU - Zhang, Kai
AU - Cui, Yanyan
AU - Guo, Yuecong
AU - Wang, Chuan
AU - Xu, Chao
AU - Yao, Qingqiang
AU - Zhao, Yuliang
AU - Chen, Chunying
AU - Wang, Yaling
N1 - Funding Information:
This work was supported by the Program for International S&T Cooperation Projects of the Ministry of Science and Technology of China (2018YFE0117200), the National Basic Research Program of China (2022YFA1603700, 2021YFA1200900, and 2021YFA1600800), Strategic Priority Research Program of Chinese Academy of Sciences (XDB36000000), and the National Natural Science Foundation of China (11621505 and 22027810); the institutionalized scientific research platform relies on Beijing Synchrotron Radiation Facility of Chinese Academy of Sciences; CAMS Innovation Fund for Medical Sciences (CIFMS 2019-I2M-5-018); the Research and Development Project in Key Areas of Guangdong Province (2019B090917011); Key-Area Research and Development Program of Guangdong Province for Guangdong high level Innovation Research Institute (2020B0909010001); the Academic Promotion Program of Shandong First Medical University (2019LJ003); the China Postdoctoral Science Foundation (2022M711974); and the Youth Science Foundation of Shandong First Medical University & Shandong Academy of Medical Sciences (202201-003).
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2023/1/31
Y1 - 2023/1/31
N2 - Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.
AB - Three-dimensional nondestructive, nanoresolution, and in situ visualization of protein spatial localization in a large, thick single cell remains challenging. In this study, we designed a multifunctional iron oxide (Fe@BFK) nanoprobe that possesses fluorescence and hard X-ray imaging signals. This probe can specifically target the human epidermal growth factor receptor 2 (HER2) protein and help optimize the label condition and selection of suitable samples for X-ray imaging. Combining 30 nm resolution synchrotron radiation hard X-ray nanocomputed tomography and the X-ray-sensitive Fe@BFK nanoprobe, a 3D localization of HER2 on SK-BR-3 cells was obtained for the first time. HER2 was mainly localized and cluster-distributed on the cell membrane with a heterogeneous pattern. This study provides a novel method for the in situ and nondestructive synchrotron radiation imaging of the desired protein localization in large, thick cells and evaluation of the true cellular distribution of a nanoprobe with high resolution.
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U2 - 10.1021/acs.analchem.2c03699
DO - 10.1021/acs.analchem.2c03699
M3 - Article
AN - SCOPUS:85145462578
VL - 95
SP - 2129
EP - 2133
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 4
ER -