TY - JOUR
T1 - Multichannel Pulsed Doppler Signal Processing for Vascular Measurements in Mice
AU - Reddy, Anilkumar K.
AU - Madala, Sridhar
AU - Jones, Alan D.
AU - Caro, Walter A.
AU - Eberth, John F.
AU - Pham, Thuy T.
AU - Taffet, George
AU - Hartley, Craig J.
N1 - Funding Information:
The authors thank Drs. Humphrey, Wilson and Gleason for allowing them to use their laboratory and equipment at Texas A&M University. The authors also thank Dr. Wehrens at the Department of Molecular Biology, Baylor College of Medicine for allowing them to use his laboratory and equipment and Dr. Chelu for helping with pacing experiments. They acknowledge Chidi Uzoki, Jennifer S. Pocius, Poornima Yechoor, Deepak Acharya and Ross Hartley for their technical contributions and Jim Brooks for his editorial review. This work was supported in part by National Institute of Health Grants R01-HL22512, R01-AG17899, R41-HL76928 and K-HL73041.
PY - 2009/12
Y1 - 2009/12
N2 - The small size, high heart rate and small tissue displacement of a mouse require small sensors that are capable of high spatial and temporal tissue displacement resolutions and multichannel data acquisition systems with high sampling rates for simultaneous measurement of high fidelity signals. We developed and evaluated an ultrasound-based mouse vascular research system (MVRS) that can be used to characterize vascular physiology in normal, transgenic, surgically altered and disease models of mice. The system consists of multiple 10/20 MHz ultrasound transducers, analog electronics for Doppler displacement and velocity measurement, signal acquisition and processing electronics and personal computer based software for real-time and off-line analysis. In vitro testing of the system showed that it is capable of measuring tissue displacement as low as 0.1 μm and tissue velocity (μm/s) starting from 0. The system can measure blood velocities up to 9 m/s (with 10 MHz Doppler at a PRF of 125 kHz) and has a temporal resolution of 0.1 milliseconds. Ex vivo tracking of an excised mouse carotid artery wall using our Doppler technique and a video pixel tracking technique showed high correlation (R2 = 0.99). The system can be used to measure diameter changes, augmentation index, impedance spectra, pulse wave velocity, characteristic impedance, forward and backward waves, reflection coefficients, coronary flow reserve and cardiac motion in murine models. The system will facilitate the study of mouse vascular mechanics and arterial abnormalities resulting in significant impact on the evaluation and screening of vascular disease in mice. (E-mail: [email protected]).
AB - The small size, high heart rate and small tissue displacement of a mouse require small sensors that are capable of high spatial and temporal tissue displacement resolutions and multichannel data acquisition systems with high sampling rates for simultaneous measurement of high fidelity signals. We developed and evaluated an ultrasound-based mouse vascular research system (MVRS) that can be used to characterize vascular physiology in normal, transgenic, surgically altered and disease models of mice. The system consists of multiple 10/20 MHz ultrasound transducers, analog electronics for Doppler displacement and velocity measurement, signal acquisition and processing electronics and personal computer based software for real-time and off-line analysis. In vitro testing of the system showed that it is capable of measuring tissue displacement as low as 0.1 μm and tissue velocity (μm/s) starting from 0. The system can measure blood velocities up to 9 m/s (with 10 MHz Doppler at a PRF of 125 kHz) and has a temporal resolution of 0.1 milliseconds. Ex vivo tracking of an excised mouse carotid artery wall using our Doppler technique and a video pixel tracking technique showed high correlation (R2 = 0.99). The system can be used to measure diameter changes, augmentation index, impedance spectra, pulse wave velocity, characteristic impedance, forward and backward waves, reflection coefficients, coronary flow reserve and cardiac motion in murine models. The system will facilitate the study of mouse vascular mechanics and arterial abnormalities resulting in significant impact on the evaluation and screening of vascular disease in mice. (E-mail: [email protected]).
KW - Arterial wall motion
KW - Doppler displacement
KW - Mouse vascular mechanics
KW - Multichannel high-frequency pulsed Doppler
KW - Pulse wave velocity
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U2 - 10.1016/j.ultrasmedbio.2009.06.1096
DO - 10.1016/j.ultrasmedbio.2009.06.1096
M3 - Article
C2 - 19854566
AN - SCOPUS:71749090210
SN - 0301-5629
VL - 35
SP - 2042
EP - 2054
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 12
ER -