Multicentre surveillance of antimicrobial resistance in enterococci and staphylococci from Colombian hospitals, 2001-2002

C. A. Arias, J. Reyes, M. Zúñiga, L. Cortés, C. Cruz, C. L. Rico, D. Panesso, Mónica Gutiérrez, Nohra de Merino, Gloria Pacheco, Otto Sussman, Diana Moncada, Ruth Quevedo, Patricia Arroyo, Martha Garzón, Carlos Saavedra, Amparo de Otero, Oscar Martínez, Edilma Torrado, Pablo Tobón UribeJaime López, Mauricio Rodríguez, Luis Angel Villar, Luz M. Gallardo, Ernesto Martínez, Ma del Pilar Crespo, Juan D. Velez, Beatriz Vanegas, Maria V. Villegas, Gloria Cardona, Carlos Saavedra, Mario Mendoza, Carlos Perez

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Invasive isolates of staphylococci and enterococci were collected from 15 tertiary care centres in live Colombian cities from 2001 to 2002. A total of 597 isolates were available for analysis. Identification was confirmed by both automated methods and multiplex PCR assays in a central laboratory. Staphylococcus aureus and coagulase-negative staphylococci (CoNS) corresponded to 49.6% and 29.6% of isolates, respectively, and 20.8% were identified as enterococci. MICs of ampicillin, ciprofloxacin, chloramphenicol, erythromycin, gentamicin, linezolid, oxacillin, rifampicin, teicoplanin, tetracycline, trimethoprim/sulfamethoxazole (SXT) and vancomycin were determined using an agar dilution method as appropriate. Screening for vancomycin-resistant S. aureus was also carried out on brain-heart infusion agar plates supplemented with vancomycin. The presence of mecA and van genes was investigated in methicillin-resistant staphylococci and glycopeptide-resistant enterococci (GRE), respectively. All staphylococci were susceptible to vancomycin, teicoplanin and linezolid. No VISA isolates were found. In S. aureus and CoNS, the lowest rates of resistance were found for SXT (7.4%) and chloramphenicol (10.7%), respectively. Resistance to oxacillin in S. aureus and CoNS was 52% and 73%, respectively. The mecA gene was detected in 97.5% of methicillin-resistant S. aureus isolates. In enterococci, resistance to glycopeptides was 9.7%: vanA (58.3%) and vanB (41.7%) genes were found. Pulsed-field gel electrophoresis indicated that the GRE isolates were closely related. Rates of resistance to ampicillin, ciprofloxacin, chloramphenicol, rifampicin and high levels of gentamicin and streptomycin were 9.7%, 27.4%, 8.9%, 43%, 17% and 28.2%, respectively. All enterococci were susceptible to linezolid.

Original languageEnglish (US)
Pages (from-to)59-68
Number of pages10
JournalJournal of Antimicrobial Chemotherapy
Volume51
Issue number1
DOIs
StatePublished - Jan 1 2003

Keywords

  • Colombia
  • Enterococci
  • Resistance
  • Staphylococci

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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