TY - JOUR
T1 - Multi-biofunctional graphene oxide-enhanced poly-L-lactic acid composite nanofiber scaffolds for ovarian function recovery of transplanted-tissue
AU - Yan, Liang
AU - Wang, Lingjuan
AU - Wu, Jiachen
AU - Wu, Yuanzheng
AU - Zhu, Xianyu
AU - Mei, Qiaojuan
AU - Song, Yinhua
AU - Liu, Yang
AU - Zhang, Ling
AU - Ai, Jihui
AU - Li, Kezhen
AU - Qing, Guangming
AU - Zhang, Yong
AU - Xiao, Xianjin
AU - Zhao, Yuliang
AU - Xiang, Wenpei
N1 - Funding Information:
We acknowledge the CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences for design and production of GO/PLLA nanofiber scaffolds. We thank Liang Yan and Yuanzheng Wu for the verification of nanofiber scaffolds properties. We thank Lingjuan Wang and Jiachen Wu for their invaluable contributions in the verification of material safety and effectiveness and manuscript writing. This work was supported by four projects listed below: National Natural Science Foundation of China (92068120), The Key R&D Program of Hubei Province (2020BCB024), China Postdoctoral Science Foundation (2020M682423), National Natural Science Foundation of China (32071403). The funders played no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
We acknowledge the CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety of Chinese Academy of Sciences for design and production of GO/PLLA nanofiber scaffolds. We thank Liang Yan and Yuanzheng Wu for the verification of nanofiber scaffolds properties. We thank Lingjuan Wang and Jiachen Wu for their invaluable contributions in the verification of material safety and effectiveness and manuscript writing. This work was supported by four projects listed below: National Natural Science Foundation of China (92068120), The Key R&D Program of Hubei Province (2020BCB024), China Postdoctoral Science Foundation (2020M682423), National Natural Science Foundation of China (32071403). The funders played no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - In this study, we successfully constructed the new graphene oxide/poly-L-lactic acid (GO/PLLA) nanofiber scaffolds with a hydrophilic surface and porous network structure that were highly favorable for cell infiltration. When employed these new nanofiber scaffolds for a wide range of tissue engineering applications, it was expected to promote graft tissue survival and angiogenesis. The new GO/PLLA nanofiber scaffold with an appropriate concentration of 1.0 wt% was applied for the restoration of ovarian function and reserve in mice with primary ovarian insufficiency (POI). After co-transplanting the normal ovarian cortex loaded on these new nanomaterials into the in situ ovarian tissue of POI mice, the fusion of transplanted ovarian cortex with damaged ovarian tissue was improved, as well as the ovarian function and the follicle numbers. Moreover, angiogenesis was observed clearly and proved to exist in the transplanted tissue and nanomaterials, with the most conspicuous effect after co-transplantation with 1.0 wt% GO/PLLA nanofiber scaffold. In addition, nitric oxide (NO) production by phosphorylated endothelial nitric oxide synthase (p-eNOS) in vivo was proven to be involved in the effect of GO and PLLA on the improved survival rate of the transplanted ovarian cortex. This study provides a new method for the fertility preservation of ovarian tissue cryopreservation and transplantation, as well as a new strategy for the transplantation of other organs.
AB - In this study, we successfully constructed the new graphene oxide/poly-L-lactic acid (GO/PLLA) nanofiber scaffolds with a hydrophilic surface and porous network structure that were highly favorable for cell infiltration. When employed these new nanofiber scaffolds for a wide range of tissue engineering applications, it was expected to promote graft tissue survival and angiogenesis. The new GO/PLLA nanofiber scaffold with an appropriate concentration of 1.0 wt% was applied for the restoration of ovarian function and reserve in mice with primary ovarian insufficiency (POI). After co-transplanting the normal ovarian cortex loaded on these new nanomaterials into the in situ ovarian tissue of POI mice, the fusion of transplanted ovarian cortex with damaged ovarian tissue was improved, as well as the ovarian function and the follicle numbers. Moreover, angiogenesis was observed clearly and proved to exist in the transplanted tissue and nanomaterials, with the most conspicuous effect after co-transplantation with 1.0 wt% GO/PLLA nanofiber scaffold. In addition, nitric oxide (NO) production by phosphorylated endothelial nitric oxide synthase (p-eNOS) in vivo was proven to be involved in the effect of GO and PLLA on the improved survival rate of the transplanted ovarian cortex. This study provides a new method for the fertility preservation of ovarian tissue cryopreservation and transplantation, as well as a new strategy for the transplantation of other organs.
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U2 - 10.1038/s41536-022-00236-5
DO - 10.1038/s41536-022-00236-5
M3 - Article
AN - SCOPUS:85138151359
VL - 7
JO - npj Regenerative Medicine
JF - npj Regenerative Medicine
SN - 2057-3995
IS - 1
M1 - 52
ER -