Abstract
The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding.
| Original language | English (US) |
|---|---|
| Article number | e4774 |
| Pages (from-to) | e4774 |
| Journal | Bio-protocol |
| Volume | 13 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jul 5 2023 |
Keywords
- BoxC/D
- Cancer immunotherapy
- Outer membrane vesicles
- RNA binding protein
- Rapid display
- mRNA vaccines
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
- Plant Science
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