Mouse model of intracerebellar haemorrhage

Abubakar Tijjani Salihu, Sangu Muthuraju, Abdul Aziz Mohamed Yusoff, Farizan Ahmad, Mohd Zulkifli Mustafa, Hasnan Jaafar, Zamzuri Idris, Abdul Rahman Izaini Ghani, Jafri Malin Abdullah

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The present study aimed to investigate the behavior and neuronal morphological changes in the perihaemorrhagic tissue of the mouse intracerebellar haemorrhage experimental model. Adult male Swiss albino mice were stereotactically infused with collagenase type VII (0.4 U/μl of saline) unilaterally in to the cerebellum, following anaesthesia. Motor deficits were assessed using open field and composite score for evaluating the mouse model of cerebellar ataxia at 1, 3, 7, 14 and 21 days after collagenase infusion. The animals were sacrificed at the same time interval for evaluation of perihaematomal neuronal degeneration using haematoxylin and eosin staining and Annexin V-FITC/Propidium iodide assay. At the end of the study, it was found that infusion of 0.4 U collagenase produces significant locomotor and ataxic deficit in the mice especially within the first week post surgery, and that this gradually improved within three weeks. Neuronal degeneration evident by cytoplasmic shrinkage and nuclear pyknosis was observed at the perihaematomal area after one day; especially at 3 and 7 days post haemorrhage. By 21 days, both the haematoma and degenerating neurons in the perihaematomal area were phagocytosed and the remaining neuronal cells around the scar tissue appeared normal. Moreover, Annexin-V/propidium iodide–positive cells were observed at the perihaematomal area at 3 and 7 days implying that the neurons likely die via apoptosis. It was concluded that a population of potentially salvageable neurons exist in the perihaematomal area after cerebellar haemorrhage throughout a wide time window that could be amenable to treatment.

Original languageEnglish (US)
Pages (from-to)374-384
Number of pages11
JournalBehavioural Brain Research
Volume312
DOIs
StatePublished - Oct 1 2016

Keywords

  • Collagenase type VII
  • Intracerebellar haemorrhage
  • Motor deficits
  • Neuronal degeneration
  • Perihaematoma

ASJC Scopus subject areas

  • Behavioral Neuroscience

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