Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat

L. F. Agnati, K. Fuxe, Z. Y. Yu, A. Härfstrand, S. Okret, A. C. Wikström, M. Goldstein, M. Zoli, W. Vale, J. Gustafsson

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    121 Scopus citations

    Abstract

    By means of the indirect immunoperoxide technique the corticotrophin releasing factor (CRF) and glucocorticoid receptor (GR) immunoreactive nerve cell bodies and the phenylethanolamine-N-methyltransferase (PNMT) immunoreactive nerve terminals in the paraventricular hypothalamic nucleus of the rat have been mapped out in adjacent vibratome sections (30 μm thick). By means of morphometrical analysis using a semiautomatic image analyser, it was possible to obtain density maps of CRF, GR and PNMT immunoreactive structures within the paraventricular hypothalamic nucleus. The statistical analysis by the use of correlation coefficients gives evidence that the PNMT immunoreactive nerve terminals innervate the majority of the CRF immunoreactive nerve cell bodies and that GR are located in the majority of the CRF immunoreactive neurons.

    Original languageEnglish (US)
    Pages (from-to)147-152
    Number of pages6
    JournalNeuroscience Letters
    Volume54
    Issue number2-3
    DOIs
    StatePublished - 1985

    Keywords

    • corticotrophin releasing factor
    • glucocorticoid receptor
    • hypothalamus
    • morphometry
    • phenylethanolamine-N-methyltransferase
    • rat

    ASJC Scopus subject areas

    • General Neuroscience

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