Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat

L. F. Agnati; K. Fuxe; Z. Y. Yu; A. Härfstrand; S. Okret; A. C. Wikström; M. Goldstein; M. Zoli; W. Vale; J. Gustafsson

doi:10.1016/S0304-3940(85)80070-7

Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat

L. F. Agnati, K. Fuxe, Z. Y. Yu, A. Härfstrand, S. Okret, A. C. Wikström, M. Goldstein, M. Zoli, W. Vale, J. Gustafsson

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

By means of the indirect immunoperoxide technique the corticotrophin releasing factor (CRF) and glucocorticoid receptor (GR) immunoreactive nerve cell bodies and the phenylethanolamine-N-methyltransferase (PNMT) immunoreactive nerve terminals in the paraventricular hypothalamic nucleus of the rat have been mapped out in adjacent vibratome sections (30 μm thick). By means of morphometrical analysis using a semiautomatic image analyser, it was possible to obtain density maps of CRF, GR and PNMT immunoreactive structures within the paraventricular hypothalamic nucleus. The statistical analysis by the use of correlation coefficients gives evidence that the PNMT immunoreactive nerve terminals innervate the majority of the CRF immunoreactive nerve cell bodies and that GR are located in the majority of the CRF immunoreactive neurons.

Original language	English (US)
Pages (from-to)	147-152
Number of pages	6
Journal	Neuroscience Letters
Volume	54
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(85)80070-7
State	Published - 1985

Keywords

corticotrophin releasing factor
glucocorticoid receptor
hypothalamus
morphometry
phenylethanolamine-N-methyltransferase
rat

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(85)80070-7

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Agnati, L. F., Fuxe, K., Yu, Z. Y., Härfstrand, A., Okret, S., Wikström, A. C., Goldstein, M., Zoli, M., Vale, W., & Gustafsson, J. (1985). Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat. Neuroscience Letters, 54(2-3), 147-152. https://doi.org/10.1016/S0304-3940(85)80070-7

Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat. / Agnati, L. F.; Fuxe, K.; Yu, Z. Y. et al.

In: Neuroscience Letters, Vol. 54, No. 2-3, 1985, p. 147-152.

Research output: Contribution to journal › Article › peer-review

Agnati, LF, Fuxe, K, Yu, ZY, Härfstrand, A, Okret, S, Wikström, AC, Goldstein, M, Zoli, M, Vale, W & Gustafsson, J 1985, 'Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat', Neuroscience Letters, vol. 54, no. 2-3, pp. 147-152. https://doi.org/10.1016/S0304-3940(85)80070-7

Agnati LF, Fuxe K, Yu ZY, Härfstrand A, Okret S, Wikström AC et al. Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat. Neuroscience Letters. 1985;54(2-3):147-152. https://doi.org/10.1016/S0304-3940(85)80070-7

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title = "Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat",

abstract = "By means of the indirect immunoperoxide technique the corticotrophin releasing factor (CRF) and glucocorticoid receptor (GR) immunoreactive nerve cell bodies and the phenylethanolamine-N-methyltransferase (PNMT) immunoreactive nerve terminals in the paraventricular hypothalamic nucleus of the rat have been mapped out in adjacent vibratome sections (30 μm thick). By means of morphometrical analysis using a semiautomatic image analyser, it was possible to obtain density maps of CRF, GR and PNMT immunoreactive structures within the paraventricular hypothalamic nucleus. The statistical analysis by the use of correlation coefficients gives evidence that the PNMT immunoreactive nerve terminals innervate the majority of the CRF immunoreactive nerve cell bodies and that GR are located in the majority of the CRF immunoreactive neurons.",

keywords = "corticotrophin releasing factor, glucocorticoid receptor, hypothalamus, morphometry, phenylethanolamine-N-methyltransferase, rat",

author = "Agnati, {L. F.} and K. Fuxe and Yu, {Z. Y.} and A. H{\"a}rfstrand and S. Okret and Wikstr{\"o}m, {A. C.} and M. Goldstein and M. Zoli and W. Vale and J. Gustafsson",

year = "1985",

doi = "10.1016/S0304-3940(85)80070-7",

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AU - Agnati, L. F.

AU - Fuxe, K.

AU - Yu, Z. Y.

AU - Härfstrand, A.

AU - Okret, S.

AU - Wikström, A. C.

AU - Goldstein, M.

AU - Zoli, M.

AU - Vale, W.

AU - Gustafsson, J.

PY - 1985

Y1 - 1985

N2 - By means of the indirect immunoperoxide technique the corticotrophin releasing factor (CRF) and glucocorticoid receptor (GR) immunoreactive nerve cell bodies and the phenylethanolamine-N-methyltransferase (PNMT) immunoreactive nerve terminals in the paraventricular hypothalamic nucleus of the rat have been mapped out in adjacent vibratome sections (30 μm thick). By means of morphometrical analysis using a semiautomatic image analyser, it was possible to obtain density maps of CRF, GR and PNMT immunoreactive structures within the paraventricular hypothalamic nucleus. The statistical analysis by the use of correlation coefficients gives evidence that the PNMT immunoreactive nerve terminals innervate the majority of the CRF immunoreactive nerve cell bodies and that GR are located in the majority of the CRF immunoreactive neurons.

AB - By means of the indirect immunoperoxide technique the corticotrophin releasing factor (CRF) and glucocorticoid receptor (GR) immunoreactive nerve cell bodies and the phenylethanolamine-N-methyltransferase (PNMT) immunoreactive nerve terminals in the paraventricular hypothalamic nucleus of the rat have been mapped out in adjacent vibratome sections (30 μm thick). By means of morphometrical analysis using a semiautomatic image analyser, it was possible to obtain density maps of CRF, GR and PNMT immunoreactive structures within the paraventricular hypothalamic nucleus. The statistical analysis by the use of correlation coefficients gives evidence that the PNMT immunoreactive nerve terminals innervate the majority of the CRF immunoreactive nerve cell bodies and that GR are located in the majority of the CRF immunoreactive neurons.

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KW - glucocorticoid receptor

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KW - morphometry

KW - phenylethanolamine-N-methyltransferase

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Morphometrical analysis of the distribution of corticotrophin releasing factor, glucocorticoid receptor and phenylethanolamine-N-methyltransferase immunoreactive structures in the paraventricular hypothalamic nucleus of the rat

Abstract

Keywords

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