TY - JOUR
T1 - Morphologic and immunohistochemical observation of otosclerotic stapes
T2 - A preliminary study
AU - Lim, David J.
AU - Saunders, William H.
N1 - Funding Information:
It is" generally believed that otosclerosis occurs only in humans, and it is considered a genetic disorder of connective tissue limited to the temporal bone. Temporal bane histopathologic studies elucidated the characteristics of this pathology (e.g., spongiotic lesion, sclerotic lesion) and its predilection sites in close association with embryonal cartilage rests in the otic capsule} While the exact underlying causes of this disorder are not yet known, numerous hypotheses have been proposed. The proposals that have been partment of Otolaryngology,O hio State UniversityC ollege of Medicine, Columbus, Ohio; and "~Providence.,R hode Island. Presented at the Workshop on Recent Advances in ate-sclerosis Research. Tenth Midwinter Research Meeting, Association for Research in Otolaryngology,F ebruary 4,1987, Clearwater Beach, Florida. Accepted for publication at that time. Supported in part by a research grant from the Research Fund of the American Otological Society. Address reprint requests to Dr. Lira: 4331 UniversityH os-pitals Clinic, 456 West 10th Avenue, Columbus, OH 43210. 0196-0709/87 $0.00 + .25 made are the following: 1) unstable cartilage rests2; 2) vascular insufficiencies3; 31 bone cell dysfunction leading to abnormal calcium metabolism, 4 release of proteolytic enzyme (e.g., lysosomal enzymes), and production of abnormal bone matrix, including collagenS'8; and 4) autoimmunity to the II collagen. 7 These hypotheses are proposed largely based on histopathologic characteristics, with the exception of the study of Paludetti et al.,4 which is based on bone-derived cells cultured from the otosclerotic stapes, and the study of Yoo et al., 7 which is based mainly on an animal experiment. Conventional temporal bone study has its limitations in that it is not suitable for elucidating the functional status of the cells involved in the pathogenesis.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1987
Y1 - 1987
N2 - Stapes with otosclerotic lesions obtained during stapedectomies were examined with light microscopy, histochemistry, immunochemistry, and electron microscopy to elucidate the cellular mechanism(s) involved in this disease process. Three types of lesions were identified: cellular (spongiotic), fibrotic, and sclerotic. The cellular type is characterized by monocyte, macrophage, osteoblast, and osteoclast recruitment and their activation. Macrophage recruitment is an early event of otosclerosis. The fibrotic type is characterized by extensive fibrosis of the bone, and the sclerotic type is characterized by a paucity of bone cells. Cytochemical results showed a large accumulation of granular substances positive for periodic acid-Schiff stain along the edge of the marrow spaces coinciding with ultrastructural calcospherite deposits, suggesting that glycosaminoglycans are involved in the mineralization process. Acid phosphatase is largely localized in the osteoclasts, but a sporadic diffusion of this enzyme was observed in the demineralizing front of the preotosclerotic lesion. Immunoglobulin G and complement C3 were colocalized in pericapillary tissue, suggesting deposit of immune complex in the spongiotic lesion. This finding indicates a possibility that immune mechanisms are involved in otosclerosis.
AB - Stapes with otosclerotic lesions obtained during stapedectomies were examined with light microscopy, histochemistry, immunochemistry, and electron microscopy to elucidate the cellular mechanism(s) involved in this disease process. Three types of lesions were identified: cellular (spongiotic), fibrotic, and sclerotic. The cellular type is characterized by monocyte, macrophage, osteoblast, and osteoclast recruitment and their activation. Macrophage recruitment is an early event of otosclerosis. The fibrotic type is characterized by extensive fibrosis of the bone, and the sclerotic type is characterized by a paucity of bone cells. Cytochemical results showed a large accumulation of granular substances positive for periodic acid-Schiff stain along the edge of the marrow spaces coinciding with ultrastructural calcospherite deposits, suggesting that glycosaminoglycans are involved in the mineralization process. Acid phosphatase is largely localized in the osteoclasts, but a sporadic diffusion of this enzyme was observed in the demineralizing front of the preotosclerotic lesion. Immunoglobulin G and complement C3 were colocalized in pericapillary tissue, suggesting deposit of immune complex in the spongiotic lesion. This finding indicates a possibility that immune mechanisms are involved in otosclerosis.
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U2 - 10.1016/S0196-0709(87)80047-9
DO - 10.1016/S0196-0709(87)80047-9
M3 - Article
C2 - 2829642
AN - SCOPUS:0023631346
VL - 8
SP - 282
EP - 295
JO - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
JF - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
SN - 0196-0709
IS - 5
ER -