Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning

Research output: Contribution to journalArticle

Mario A. Torres-Acosta, Suria I. Morales-Guzman, Federico Ruiz-Ruiz, Patricia Vazquez-Villegas, Richard C. Willson, Marco Rito-Palomares

BACKGROUND: Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two-phase systems (ATPS) and three-phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS: TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t-butanol hinders its acceptance. CONCLUSION: Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs.

Original languageEnglish (US)
Pages (from-to)2511-2517
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Volume93
Issue number9
DOIs
StatePublished - Sep 1 2018

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Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning. / Torres-Acosta, Mario A.; Morales-Guzman, Suria I.; Ruiz-Ruiz, Federico; Vazquez-Villegas, Patricia; Willson, Richard C.; Rito-Palomares, Marco.

In: Journal of Chemical Technology and Biotechnology, Vol. 93, No. 9, 01.09.2018, p. 2511-2517.

Research output: Contribution to journalArticle

Harvard

Torres-Acosta, MA, Morales-Guzman, SI, Ruiz-Ruiz, F, Vazquez-Villegas, P, Willson, RC & Rito-Palomares, M 2018, 'Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning' Journal of Chemical Technology and Biotechnology, vol. 93, no. 9, pp. 2511-2517. DOI: 10.1002/jctb.5730

APA

Torres-Acosta, M. A., Morales-Guzman, S. I., Ruiz-Ruiz, F., Vazquez-Villegas, P., Willson, R. C., & Rito-Palomares, M. (2018). Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning. Journal of Chemical Technology and Biotechnology, 93(9), 2511-2517. DOI: 10.1002/jctb.5730

Vancouver

Torres-Acosta MA, Morales-Guzman SI, Ruiz-Ruiz F, Vazquez-Villegas P, Willson RC, Rito-Palomares M. Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning. Journal of Chemical Technology and Biotechnology. 2018 Sep 1;93(9):2511-2517. Available from, DOI: 10.1002/jctb.5730

Author

Torres-Acosta, Mario A. ; Morales-Guzman, Suria I. ; Ruiz-Ruiz, Federico ; Vazquez-Villegas, Patricia ; Willson, Richard C. ; Rito-Palomares, Marco. / Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning. In: Journal of Chemical Technology and Biotechnology. 2018 ; Vol. 93, No. 9. pp. 2511-2517

BibTeX

@article{15c276600fcf458f89d8ad1a83219dae,
title = "Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning",
abstract = "BACKGROUND: Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two-phase systems (ATPS) and three-phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS: TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t-butanol hinders its acceptance. CONCLUSION: Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs.",
keywords = "aqueous two-phase systems, economic analysis, Monte Carlo simulations, optimization, sensitivity analysis, three-phase partitioning, uncertainty",
author = "Torres-Acosta, {Mario A.} and Morales-Guzman, {Suria I.} and Federico Ruiz-Ruiz and Patricia Vazquez-Villegas and Willson, {Richard C.} and Marco Rito-Palomares",
year = "2018",
month = "9",
day = "1",
doi = "10.1002/jctb.5730",
language = "English (US)",
volume = "93",
pages = "2511--2517",
journal = "Journal of Chemical Technology and Biotechnology",
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}

RIS

TY - JOUR

T1 - Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning

AU - Torres-Acosta,Mario A.

AU - Morales-Guzman,Suria I.

AU - Ruiz-Ruiz,Federico

AU - Vazquez-Villegas,Patricia

AU - Willson,Richard C.

AU - Rito-Palomares,Marco

PY - 2018/9/1

Y1 - 2018/9/1

N2 - BACKGROUND: Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two-phase systems (ATPS) and three-phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS: TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t-butanol hinders its acceptance. CONCLUSION: Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs.

AB - BACKGROUND: Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two-phase systems (ATPS) and three-phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS: TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t-butanol hinders its acceptance. CONCLUSION: Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs.

KW - aqueous two-phase systems

KW - economic analysis

KW - Monte Carlo simulations

KW - optimization

KW - sensitivity analysis

KW - three-phase partitioning

KW - uncertainty

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U2 - 10.1002/jctb.5730

DO - 10.1002/jctb.5730

M3 - Article

VL - 93

SP - 2511

EP - 2517

JO - Journal of Chemical Technology and Biotechnology

T2 - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 9

ER -

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