Monte Carlo economic analysis of Baker's yeast invertase purification using two- and three-phase partitioning

Mario A. Torres-Acosta, Suria I. Morales-Guzman, Federico Ruiz-Ruiz, Patricia Vazquez-Villegas, Richard C. Willson, Marco Rito-Palomares

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


BACKGROUND: Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two-phase systems (ATPS) and three-phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS: TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t-butanol hinders its acceptance. CONCLUSION: Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs.

Original languageEnglish (US)
Pages (from-to)2511-2517
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Issue number9
StatePublished - Sep 2018


  • Monte Carlo simulations
  • aqueous two-phase systems
  • economic analysis
  • optimization
  • sensitivity analysis
  • three-phase partitioning
  • uncertainty

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry


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