Monoclonal anti-actin antibody recognizes a surface molecule on normal and transformed human B lymphocytes: Expression varies with phase of cell cycle

Mark A. Bach, Dorothy E. Lewis, John E. McClure, Nirmi Parikh, Howard M. Rosenblatt, William T. Shearer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8-40% of LA350 cells and 3-15% of peripheral blood lymphocytes from healthy donors stain specifically with 2C9, almost exclusively on IgM-positive cells. Treatment of cells with 2C9 prior to incubation caused cell-surface actin to first patch and then to cap. Treatment of cells with nonspecific protease caused a loss of surface actin, with reexpression of the marker after 8-12 hr. The expression of LA350 surface actin also increased with DNA synthesis and was demonstrated to be maximal during late G1/early S phase. Thus, this antigen may be a sensitive marker for activated lymphocytes. These studies contribute to our understanding of the expression and distribution of actin-like membrane proteins that may participate in regulatory signals mediated by anti-actin antibody.

Original languageEnglish (US)
Pages (from-to)364-374
Number of pages11
JournalCellular Immunology
Volume98
Issue number2
DOIs
StatePublished - Apr 1 1986

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Monoclonal anti-actin antibody recognizes a surface molecule on normal and transformed human B lymphocytes: Expression varies with phase of cell cycle'. Together they form a unique fingerprint.

Cite this