Hydrogels based on 2-hydroxyethyl methacrylate (HEMA) crosslinked with tetraethylene glycol (TEGDA) and molecularly engineered using two methacrylate-based monomers, poly (ethylene glycol) (200) monomethacrylate (PEGMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) in the range of 0.0 - 0.5 mol % and 0-10 mol % respectively were investigated. Hydration studies demonstrated up to a 93.8% increase in the hydration with an increase in the MPC content Data obtained from the measurement of the fluorescence intensity of FITC-dye tagged protein adsorbed onto various hydrogel substrates when exposed to solutions of different protein concentration solutions at 25°C were modeled to the Langmuir isotherm. Variables Kd and Qm confirmed the reduction in the adsorption of protein onto hydrogels with the increase in the MPC concentration and with extensive hydration of the hydrogels, 5 days. Cell viability studies using human aortic muscle endothelial cells exhibited greater than 80% viability with all the hydrogel formulations studied. Cell retention in the hydrogel matrix was investigated by staining cells that remained in the hydrogel matrix following trypsinization, with a fluorescent dye DAPI. It was observed, using fluorescence microscopy, that the higher the MPC content in the hydrogel the greater the cell retention capacity of the hydrogel.