Molecularly engineered p(HEMA)-based hydrogels possessing poly(ethylene glycol) and phosphorylcholine for implant biocompatibility

Sheena Abraham, Anthony Guiseppi-Elie

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Hydrogels based on 2-hydroxyethyl methacrylate (HEMA) crosslinked with tetraethylene glycol (TEGDA) and molecularly engineered using two methacrylate-based monomers, poly (ethylene glycol) (200) monomethacrylate (PEGMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) in the range of 0.0 - 0.5 mol % and 0-10 mol % respectively were investigated. Hydration studies demonstrated up to a 93.8% increase in the hydration with an increase in the MPC content Data obtained from the measurement of the fluorescence intensity of FITC-dye tagged protein adsorbed onto various hydrogel substrates when exposed to solutions of different protein concentration solutions at 25°C were modeled to the Langmuir isotherm. Variables Kd and Qm confirmed the reduction in the adsorption of protein onto hydrogels with the increase in the MPC concentration and with extensive hydration of the hydrogels, 5 days. Cell viability studies using human aortic muscle endothelial cells exhibited greater than 80% viability with all the hydrogel formulations studied. Cell retention in the hydrogel matrix was investigated by staining cells that remained in the hydrogel matrix following trypsinization, with a fluorescent dye DAPI. It was observed, using fluorescence microscopy, that the higher the MPC content in the hydrogel the greater the cell retention capacity of the hydrogel.

Original languageEnglish (US)
Title of host publicationProceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4099-4102
Number of pages4
ISBN (Print)0780387406, 9780780387409
DOIs
StatePublished - 2005
Event2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
Duration: Sep 1 2005Sep 4 2005

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume7 VOLS
ISSN (Print)0589-1019

Other

Other2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
CountryChina
CityShanghai
Period9/1/059/4/05

Keywords

  • Biocompatibility
  • Cell retention
  • Cell viability
  • Hydrogels
  • Langmuir isotherm
  • Protein adsorption

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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