Molecular microbiological methods in the diagnosis of neonatal sepsis

Mohan Venkatesh, Angela Flores, Ruth Ann Luna, James Versalovic

Research output: Contribution to journalReview article

38 Scopus citations

Abstract

Neonatal sepsis is a major cause of neonatal mortality and morbidity. The current gold standard for diagnosis of sepsis, namely blood culture, suffers from low sensitivity and a reporting delay of approximately 48-72 h. Rapid detection of sepsis and institution of antimicrobial therapy may improve patient outcomes. Rapid and sensitive tests that can inform clinicians regarding the institution or optimization of antimicrobial therapy are urgently needed. The ideal diagnostic test should have adequate specificity and negative predictive value to reliably exclude sepsis and avoid unnecessary antibiotic therapy. We comprehensively searched for neonatal studies that evaluated molecular methods for diagnosis of sepsis. We identified 19 studies that were assessed with respect to assay methodology and diagnostic characteristics. In addition, we also reviewed newer molecular microbiological assays of relevance that have not been fully evaluated in neonates. Molecular methods offer distinct advantages over blood cultures, including increased sensitivity and rapid diagnosis. However, diagnostic accuracy and cost-effectiveness should be established before implementation in clinical practice.

Original languageEnglish (US)
Pages (from-to)1037-1048
Number of pages12
JournalExpert Review of Anti-Infective Therapy
Volume8
Issue number9
DOIs
StatePublished - Sep 2010

Keywords

  • diagnosis
  • FISH
  • microarray
  • molecular
  • neonate
  • PCR
  • sepsis

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology
  • Virology

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