Molecular genetic dissection of mouse unconventional myosin-VA: Tail region mutations

Jian Dong Huang, Valerie Mermall, Marjorie C. Strobel, Liane B. Russell, Mark S. Mooseker, Neal G. Copeland, Nancy A. Jenkins

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

We used an RT-PCR-based sequencing approach to identify the mutations responsible for 17 viable dilute alleles, a mouse-coat-color locus encoding unconventional myosin-VA. Ten of the mutations mapped to the MyoVA tail and are reported here. These mutations represent the first extensive collection of tail mutations reported for any unconventional mammalian myosin. They identify sequences important for tail function and identify domains potentially involved in cargo binding and/or proper folding of the MyoVA tail. Our results also provide support for the notion that different myosin tail isoforms produced by alternative splicing encode important cell-type- specific functions.

Original languageEnglish (US)
Pages (from-to)1963-1972
Number of pages10
JournalGenetics
Volume148
Issue number4
StatePublished - Apr 1998

ASJC Scopus subject areas

  • Genetics

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