Molecular evolution and host adaptation of Bordetella spp. Phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences

Anneke Van Der Zee, Frits Mooi, Jan Van Embden, James Musser

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

A total of 188 Bordetella strains were characterized by the electrophoretic mobilities of 15 metabolic enzymes and the distribution and variation in positions and copy numbers of three insertion sequences (IS). The presence or absence of IS elements within certain lineages was congruent with estimates of overall genetic relationships as revealed by multilocus enzyme electrophoresis. Bordetella pertussis and ovine B. parapertussis each formed separate clusters, while human B. parapertussis was most closely related to IS1001-containing B. bronchiseptica isolates. The results of the analysis provide support for the hypothesis that the population structure of Bordetella is predominantly clonal, with relatively little effective horizontal gene flow. Only a few examples of putative recombinational exchange of an IS element were detected. Based on the results of this study, we tried to reconstruct the evolutionary history of different host-adapted lineages.

Original languageEnglish (US)
Pages (from-to)6609-6617
Number of pages9
JournalJournal of bacteriology
Volume179
Issue number21
DOIs
StatePublished - Nov 1997

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Molecular evolution and host adaptation of Bordetella spp. Phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences'. Together they form a unique fingerprint.

Cite this