TY - JOUR
T1 - Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility
AU - Hardwick, C.
AU - Hoare, K.
AU - Owens, R.
AU - Hohn, H. P.
AU - Hook, M.
AU - Moore, D.
AU - Cripps, V.
AU - Austen, L.
AU - Nance, D. M.
AU - Turley, E. A.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1992
Y1 - 1992
N2 - A cDNA encoding a unique hyaluronan receptor has been molecularly cloned from a XGT11 3T3 cDNA expression library. Immunoblot analyses of cell lysates, using antibodies to peptides encoded in the cDNA, specifically react with a 58-kD protein. This protein is regulated by the mutant H-ras gene in cells containing a metallothionein promoter H-ras hybrid gene. Further, antibodies to peptide sequences encoded in the cDNA block the increase in locomotion resulting from induction of the mutant H-ras gene in this cell line. In a transblot assay, the bacterially expressed protein binds to biotinylated hyaluronan. Antibodies to peptides encoded in the cDNA react in immunoblot assays with the 58- and 52-kD proteins of a novel hyaluronan receptor complex previously implicated in cell locomotion. Furthermore, antibodies specific to the 58- and 52-kD proteins, which block ras-induced locomotion, also cross-react with the expressed, encoded protein. The gene product described here appears to be a new type of hyaluronan receptor that is involved in cell locomotion. It is named RHAMM, an acronym for receptor for hyaluronan-mediated motility.
AB - A cDNA encoding a unique hyaluronan receptor has been molecularly cloned from a XGT11 3T3 cDNA expression library. Immunoblot analyses of cell lysates, using antibodies to peptides encoded in the cDNA, specifically react with a 58-kD protein. This protein is regulated by the mutant H-ras gene in cells containing a metallothionein promoter H-ras hybrid gene. Further, antibodies to peptide sequences encoded in the cDNA block the increase in locomotion resulting from induction of the mutant H-ras gene in this cell line. In a transblot assay, the bacterially expressed protein binds to biotinylated hyaluronan. Antibodies to peptides encoded in the cDNA react in immunoblot assays with the 58- and 52-kD proteins of a novel hyaluronan receptor complex previously implicated in cell locomotion. Furthermore, antibodies specific to the 58- and 52-kD proteins, which block ras-induced locomotion, also cross-react with the expressed, encoded protein. The gene product described here appears to be a new type of hyaluronan receptor that is involved in cell locomotion. It is named RHAMM, an acronym for receptor for hyaluronan-mediated motility.
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U2 - 10.1083/jcb.117.6.1343
DO - 10.1083/jcb.117.6.1343
M3 - Article
C2 - 1376732
AN - SCOPUS:0026645557
VL - 117
SP - 1343
EP - 1350
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 6
ER -