Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility

C. Hardwick, K. Hoare, R. Owens, H. P. Hohn, M. Hook, D. Moore, V. Cripps, L. Austen, D. M. Nance, E. A. Turley

Research output: Contribution to journalArticle

289 Scopus citations

Abstract

A cDNA encoding a unique hyaluronan receptor has been molecularly cloned from a XGT11 3T3 cDNA expression library. Immunoblot analyses of cell lysates, using antibodies to peptides encoded in the cDNA, specifically react with a 58-kD protein. This protein is regulated by the mutant H-ras gene in cells containing a metallothionein promoter H-ras hybrid gene. Further, antibodies to peptide sequences encoded in the cDNA block the increase in locomotion resulting from induction of the mutant H-ras gene in this cell line. In a transblot assay, the bacterially expressed protein binds to biotinylated hyaluronan. Antibodies to peptides encoded in the cDNA react in immunoblot assays with the 58- and 52-kD proteins of a novel hyaluronan receptor complex previously implicated in cell locomotion. Furthermore, antibodies specific to the 58- and 52-kD proteins, which block ras-induced locomotion, also cross-react with the expressed, encoded protein. The gene product described here appears to be a new type of hyaluronan receptor that is involved in cell locomotion. It is named RHAMM, an acronym for receptor for hyaluronan-mediated motility.

Original languageEnglish (US)
Pages (from-to)1343-1350
Number of pages8
JournalJournal of Cell Biology
Volume117
Issue number6
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Cell Biology

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