Molecular cloning of a human RP105 homologue and chromosomal localization of the mouse and human RP105 genes (Ly64 and LY64)

Yoshihiro Miura, Kensuke Miyake, Yoshio Yamashita, Rintaro Shimazu, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Johji Inazawa, Tatsuo Abe, Masao Kimoto

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

RP105 is a mouse B cell surface molecule that transmits a growth- promoting signal and is implicated in the life/death decision of B cells. RP105 has tandem repeats of a leucine-rich motif in the extracellular domain that is expected to be involved in protein-protein interactions. In the present study, a cDNA clone encoding the human homologue of RP105 was isolated. The amino acid sequence of human RP105 is highly homologous to that of mouse RP105 with 74% identity, and the leucine-rich repeats are well conserved. The expression of the human RP105 transcript was detected in some B cell lines, a histiocytic leukemia cell line, and peripheral blood leukocytes. We also determined the chromosomal locations of the mouse RP105 gene (Ly64 locus) and the human RP105 gene (LY64 locus). Interspecific mouse backcross analysis was used to map the Ly64 locus at the distal region of chromosome 13. The human LY64 locus was localized to 5q12 by fluorescence in situ hybridization, confirming the syntenic relationship between these regions of the mouse and human chromosomes.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalGenomics
Volume38
Issue number3
DOIs
StatePublished - Dec 15 1996

ASJC Scopus subject areas

  • Genetics

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