Molecular cloning, expression, chromosomal assignment, and tissue-specific expression of a murine α-(1,3)-fucosyltransferase locus corresponding to the human ELAM-1 ligand fucosyl transferase

K. M. Gersten, S. Natsuka, M. Trinchera, B. Petryniak, R. J. Kelly, N. Hiraiwa, N. A. Jenkins, D. J. Gilbert, N. G. Copeland, J. B. Lowe

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Terminal Fucα2-3GlcNAc moieties are displayed by mammalian cell surface glycoconjugates in a tissue-specific manner. These oligosaccharides participate in selectin-dependent leukocyte adhesion and have been implicated in adhesive events during murine embryogenesis. Other functions for these molecules remain to be defined, as do the tissue-specific expression patterns of the corresponding α-(1-3)-fucosyltransferase (α1-3FT) genes. This report characterizes a murine α1-3FT that shares 77% amino acid sequence identity with human ELAM ligand fucosyltransferase (ELFT, also termed Fuc-TIV). The corresponding gene maps to mouse chromosome 9 in a region of homology with the Fuc-TIV locus on human chromosome 11q. In vitro, the murine α1-3FT can efficiently fucosylate the trisaccharide Galα1-3Galβ1-4GlcNAc (apparent K(m) of 0.71 mM) to form an unusual tetrasaccharide (Galα1-3Ga1β1- 4[Fucα1-3]GlcNAc) described in periimplantation mouse tissues. The enzyme can also form the Lewis x determinant from Galβ1-4GlcNAc (K(m) = 2.05 mM), and the sialyl Lewis x determinant from NeuNAcα2-3Galβ1-4GlcNAc (K(m) = 1.78 mM). However, it does not yield sialyl Lewis x determinants when expressed in a mammalian cell line that maintains sialyl Lewis x precursors. Transcripts from this gene accumulate to low levels in hematopoietic organs, but are unexpectedly abundant in epithelia that line the stomach, small intestine, colon, and epididymus. Epithelial cell-specific expression of this gene suggests function(s) in addition to, and distinct from, its proposed role in selectin ligand synthesis.

Original languageEnglish (US)
Pages (from-to)25047-25056
Number of pages10
JournalJournal of Biological Chemistry
Volume270
Issue number42
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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