Molecular Cloning and Expression of the Type 1 and Type 2 Murine Receptors for Tumor Necrosis Factor

Raymond G. Goodwin, Dirk Anderson, Rita Jerzy, Terri Davis, Camilynn I. Brannan, Neal G. Copeland, Nancy A. Jenkins, Craig A. Smith

Research output: Contribution to journalArticle

157 Scopus citations

Abstract

Clones encoding the type 1 (p80) and type 2 (p60) forms of the murine receptors for tumor necrosis factor (TNF) were isolated by cross-hybridization using probes derived from the cloned human TNF receptors. Each of the murine receptors shows strong sequence homology to the corresponding human receptor (∼65% amino acid identity) throughout the molecule but only modest homology, limited to ligand-binding domains, between themselves. The ligand-binding characteristics of the recombinant murine receptors mirror those of the human homologs: both receptor types bind TNF-α and -β with multiple affinity classes, and the ligands cross-compete. Analysis of the murine transcripts encoding these receptors revealed the presence of RNAs for one or both forms of the receptors in all cells examined. It was also demonstrated that for both types of human TNF receptor, variably sized transcripts are observed in different cells. The murine cDNAs were further used to determine the chromosomal locations of the TNF receptor genes. They are not linked, in contrast to the ligands, and map to chromosomes 4 (type 1) and 6 (type 2).

Original languageEnglish (US)
Pages (from-to)3020-3026
Number of pages7
JournalMolecular and Cellular Biology
Volume11
Issue number6
DOIs
StatePublished - Jun 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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