Molecular cloning and biological characterization of a novel murine lymphoid growth factor

John E. Sims, Douglas E. Williams, Philip J. Morrissey, Kirsten Garka, Diane Foxworthe, Virginia Price, Sherree L. Friend, Andrew Farr, Mary A. Bedell, Nancy A. Jenkins, Neal G. Copeland, Kenneth Grabstein, Raymond J. Paxton

Research output: Contribution to journalArticle

178 Scopus citations

Abstract

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220+BP-1+ pre-B cells.

Original languageEnglish (US)
Pages (from-to)671-680
Number of pages10
JournalJournal of Experimental Medicine
Volume192
Issue number5
DOIs
StatePublished - Sep 4 2000

Keywords

  • B lymphocyte
  • Cytokine
  • DNA sequence
  • Growth factor
  • Lymphocyte differentiation

ASJC Scopus subject areas

  • Immunology

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