Molecular characterization of an invasive phenotype of group a streptococcus arising during human infection using whole genome sequencing of multiple isolates from the same patient

Anthony R. Flores, Pranoti Sahasrabhojane, Miguel Saldaña, Jessica Galloway-Peña, Randall J. Olsen, James M. Musser, Samuel Shelburne

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Invasive group A streptococcal (GAS) strains often have genetic differences compared to GAS strains from nonsterile sites. Invasive, " hypervirulent" GAS strains can arise from a noninvasive progenitor following subcutaneous inoculation in mice, but such emergence has been rarely characterized in humans. We used whole genome analyses of multiple GAS isolates from the same patient to document the molecular basis for emergence of a GAS strain with an invasive phenotype during human infection. In contrast to previous theories, we found that elimination of production of the cysteine protease SpeB was not necessary for emergence of GAS with an invasive, "hypervirulent" phenotype.

Original languageEnglish (US)
Pages (from-to)1520-1523
Number of pages4
JournalJournal of Infectious Diseases
Volume209
Issue number10
DOIs
StatePublished - May 15 2014

Keywords

  • bacterial pathogenesis
  • gene expression.
  • group A Streptococcus
  • virulence
  • whole genome sequencing

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

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