Molecular characterization and phylogenetic analysis of a novel glutenin gene (Dy10.1t) from Aegilops tauschii

Yanzhen Zhang, Qiaoyun Li, Yueming Yan, Jigang Zheng, Xueli An, Yinghua Xiao, Aili Wang, Yuhe Pei, Haibo Wang, Sai L.K. Hsam, Friedrich J. Zeller

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A novel y-type high molecular mass glutenin subunit (HMM-GS) possessing a mobility that is slightly slower than that of the subunit Dy10 obtained by SDS-PAGE, named Dy10.1t, in the wild wheat Aegilops tauschii was identified by 1- and 2-dimensional gel electrophoresis, capillary electrophoresis, and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The gene encoding the HMM subunit Dy10.1 t was amplified with allele-specific PCR primers, and the amplified products were cloned and sequenced. The coding domain of the Dy10.1t subunit gene consisted of 1980 bp encoding a protein of 658 residues with an Mrs of 68 611 Da, which was similar to the Mrs determined by MALDI-TOF-MS. The deduced amino acid sequence indicated that Dy10.1 t subunit displayed a greater similarity to the Dy12 subunit, differing by only 8 amino acid substitutions. Six coding region single-nucleotide polymorphisms were discovered in the Dy10.1t gene by multiple alignments (1 per 330 bp), 1 in the N-terminal domain and the others in the central repeats. Five of them resulted in residue substitutions, whereas 3 created enzyme site changes. The homology and neighbour-joining trees constructed from code domain sequences of 20 x-and y-type glutenin genes from different Triticum species separated into 2 halves, which corresponded to the x-type and y-type HMM glutenin alleles. Phylogenetic analysis revealed that the Glu-1 gene duplication event probably occurred at about 16.83 million years ago, whereas the divergence times of A, B, and D genomes within x-type and y-type halves were before 7.047 and 10.54 million years ago, respectively.

Original languageEnglish (US)
Pages (from-to)735-745
Number of pages11
JournalGenome
Volume49
Issue number7
DOIs
StatePublished - Jul 1 2006

Keywords

  • AS-PCR
  • Aegilops tauschii
  • HMW glutenin genes
  • MALDI-TOF-MS
  • Phylogenetic analysis
  • cSNP

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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