Molecular characterization and mapping of murine genes encoding three members of the stefin family of cysteine proteinase inhibitors

Florence W.L. Tsui, Hing Wo Tsui, Samuel Mok, Irena Mlinaric, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Katherine A. Siminovitch

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Stefins or Type 1 cystatins belong to a large, evolutionarily conserved protein superfamily, the members of which inhibit the papain-like cysteine proteinases. We report here on the molecular cloning and chromosomal localization of three newly identified members of the murine stefin gene family. These genes, designated herein as mouse stefins 1, 2, and 3, were isolated on the basis of their relatively increased expression in motheaten viable compared to normal congenic mouse bone marrow cells. The open reading frames of the stefin cDNAs encode proteins of approximately 11.5 kDa that show between 50 and 92% identity to sequences of stefins isolated from various other species. Data from Southern analysis suggest that the murine stefin gene family encompasses at least 6 and possibly 10-20 members, all of which appear to be clustered in the genome. Analysis of interspecific backcross mice indicates that the genes encoding the three mouse stefins all map to mouse chromosome 16, a localization that is consistent with the recent assignment of the human stefin A gene to a region of conserved homology between human chromosome 3q and the proximal region of mouse chromosome 16.

Original languageEnglish (US)
Pages (from-to)507-514
Number of pages8
JournalGenomics
Volume15
Issue number3
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Molecular characterization and mapping of murine genes encoding three members of the stefin family of cysteine proteinase inhibitors'. Together they form a unique fingerprint.

Cite this