Molecular basis for pseudo vitamin D-deficiency rickets in the Hannover pig

L. S. Chavez, Rita Serda, S. Choe, L. Davidi, J. Harmeyer, J. L. Omdahl

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The molecular basis for pseudo vitamin D deficiency rickets (PDDR) in the Hannover pig model was determined in the current study. Consistent with the inability of Hannover PDDR pigs to maintain ambient levels of 1,25-dihydroxyvitamin D (i.e., 1,25D), the bioactivation enzyme cytochrome P450C1 (or CYP27B1) was determined to contain coding-region deletions that rendered the enzyme ineffective due to frame-shift mutations and expression of a premature termination codon. Expression levels of P450C1mRNA were up-regulated in response to the low-1,25D high-parathyroid hormone state of the PDDR animals. In a complementary manner, cytochrome P450C24 mRNA was not detectable in PDDR pigs. Two different deletions were detected within the Hannover pig strain in which the P450C1 coding region contained either 173 bp or 329 bp deletions that resulted in the expression of non-sense products beginning within the I-helix region and extending through the truncated C-terminal domains. The boundaries for the deletion segments aligned with derived mRNA processing sites. This observation was consistent with an mRNA processing error as the causative factor for the coding-region deletions. Based upon the expression of a non-functional P450C1 enzyme, the Hannover pig model for PDDR was determined to be identical to the human disease in which enzyme-inhibitory mutations are the molecular basis for the calcium disorder.

Original languageEnglish (US)
Pages (from-to)378-385
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume14
Issue number7
DOIs
StatePublished - Jul 1 2003

Keywords

  • Mutation
  • Pig
  • Rickets
  • RNA deletion
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Molecular basis for pseudo vitamin D-deficiency rickets in the Hannover pig'. Together they form a unique fingerprint.

Cite this