Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells

Junji Xing, Fuqing Wu, Weiwei Pan, Chunfu Zheng

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The herpes simplex virus type I (HSV-1) US11 protein is an RNA-binding multifunctional regulator that specifically and stably associates with nucleoli. Although the C-terminal part of US11 was responsible for its nucleolar localization, the precise nucleolar localization signal (NoLS) and nuclear export signal (NES) of US11 and its nuclear import and export mechanisms are still elusive. In this study, fluorescence microscopy was employed to investigate the subcellular localization of US11 and characterize its transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein (EYFP), three novel NoLSs of US11 were for the first time mapped to amino acids 84-125, 126-152, and 89-146, respectively. Additionally, the NES was identified to locate between amino acids 89 and 119. Furthermore, the US11 protein was demonstrated to target to the cytoplasm through the NES by chromosomal region maintenance 1 (CRM1)-independent pathway, and to the nucleolus through Ran and importin β-dependent mechanism that does not require importin α5.

Original languageEnglish (US)
Pages (from-to)71-81
Number of pages11
JournalVirus Research
Volume153
Issue number1
DOIs
StatePublished - Oct 2010

Keywords

  • Herpes simplex virus 1 US11 protein
  • Importin
  • Nuclear export signal
  • Nucleolar localization signal
  • Ran-GTP

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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