TY - JOUR
T1 - Modulation of adenoviral transduction in vitro and in vivo by hyaluronan and its receptor CD44
AU - Chaudhuri, Saumya R.
AU - Mallam, Joshua N.
AU - Chévez-Barrios, Patricia
AU - Wadhwa, Lalita
AU - Ng, Philip
AU - Hurwitz, Mary Y.
AU - Hurwitz, Richard
N1 - Funding Information:
This research was supported by Grants from the Clayton Foundation for Research, the Retina Research Foundation, and the Knights Templar Eye Foundation. The technical assistance of Karen T Marcus, Vien Holcombe (Department of Pediatrics, Baylor College of Medicine), and Donna J Palmer (Department of Molecular and Human Genetics, Baylor College of Medicine), and the critical review of this paper by Malcolm Brenner (Center for Cell and Gene Therapy, Baylor College of Medicine) are gratefully acknowledged.
PY - 2007/3
Y1 - 2007/3
N2 - Adenovirus infection is a significant cause of ocular, respiratory, and gastrointestinal illness and can spread rapidly. Morbidity is considerable in immune-suppressed individuals and there is significant mortality. There are no effective therapies. During preclinical studies of adenoviral-mediated gene therapy for ocular disorders, we noticed a significant increase in transduction when the target cells were exposed to adenovirus in the presence of ocular vitreous. The vitreous is mainly comprised of water, collagen, and the large polysaccharide hyaluronan. In this paper, we report data that implicate hyaluronan in the adenoviral infectious process and show that interference with the interaction between hyaluronan and its cellular receptor CD44 can block adenovirus transduction in vitro and in vivo.
AB - Adenovirus infection is a significant cause of ocular, respiratory, and gastrointestinal illness and can spread rapidly. Morbidity is considerable in immune-suppressed individuals and there is significant mortality. There are no effective therapies. During preclinical studies of adenoviral-mediated gene therapy for ocular disorders, we noticed a significant increase in transduction when the target cells were exposed to adenovirus in the presence of ocular vitreous. The vitreous is mainly comprised of water, collagen, and the large polysaccharide hyaluronan. In this paper, we report data that implicate hyaluronan in the adenoviral infectious process and show that interference with the interaction between hyaluronan and its cellular receptor CD44 can block adenovirus transduction in vitro and in vivo.
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U2 - 10.1038/sj.mt.6300044
DO - 10.1038/sj.mt.6300044
M3 - Article
C2 - 17180120
AN - SCOPUS:33847200462
SN - 1525-0016
VL - 15
SP - 566
EP - 570
JO - Molecular Therapy
JF - Molecular Therapy
IS - 3
ER -