Modulation of acetylcholine release in rat hippocampus by amino alcohols and Bay K 8644

J. Robert Bostwick, Russell Abbe, Stanley H. Appel

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The amino alcohols ethanolamine, R-alaninol and R-prolinol were shown to enhance high potassium evoked release of [3H]acetylcholine from hippocampal slices by monitoring fractional release of tritium during superfusion. This action appeared to be unique to hippocampal cholinergic nerve terminals because R-prolinol did not modulate evoked release acetylcholine from cortical or striatal slices, dopamine from striatal slices or norepinephrine from hippocampal slices. Bay K 8644, a dihydropyridine activator of calcium L-channels, exhibited a similar specificity profile. Bay K 8644 decreased the EC50 of R-prolinol without changing the maximal response, indicating that the actions of these two compounds converge through a common cellular mechanism. The effect of R-prolinol was blocked by the L-channel antagonists diltiazem and verapamil but not by nifedipine. In contrast, nifedipine only and not diltiazem or verapamil, blocked the enhancement induced by Bay K 8644. It appears then that amino alcohols can modulate the release of acetylcholine in the hippocampus possibly by enhancing calcium entry into nerve terminals through a specific activation of presynaptic L-channels at a site other than that which interacts with dihydropyridines.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalBrain Research
Issue number1
StatePublished - Nov 26 1993


  • Calcium channel
  • Diltiazem
  • Ethanolamine
  • Neurotransmitter release
  • R-Prolinol

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology
  • Developmental Biology


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