Modified titanium surface with gelatin nano gold composite increases osteoblast cell biocompatibility

Young Hee Lee, Govinda Bhattarai, Santosh Aryal, Nan Hee Lee, Min Ho Lee, Tae Gun Kim, Eun Chung Jhee, Hak Yong Kim, Ho Keun Yi

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


This study examined the gelatin nano gold (GnG) composite for surface modification of titanium in addition to insure biocompatibility on dental implants or biomaterials. The GnG composite was constructed by gelatin and hydrogen tetrachloroaurate in presence of reducing agent, sodium borohydrate (NabH4). The GnG composite was confirmed by UV-VIS spectroscopy and transmission electron microscopy (TEM). A dipping method was used to modify the titanium surface by GnG composite. Surface was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The MC-3T3 E1 cell viability was assessed by trypan blue and the expression of proteins to biocompatibility were analyzed by Western blotting. The GnG composite showed well dispersed character, the strong absorption at 530 nm, roughness, regular crystal and clear C, Na, Cl, P, and Au signals onto titanium. Further, this composite allowed MC-3T3 E1 growth and viability compared to gelatin and pure titanium. It induced ERK activation and the expression of cell adherent molecules, FAK and SPARC, and growth factor, VEGF. However, GnG decreased the level of SAPK/JNK. This shows that GnG composite coated titanium surfaces have a good biocompatibility for osteoblast growth and attachment than in intact by simple and versatile dipping method. Furthermore, it offers good communication between cell and implant surfaces by regulating cell signaling and adherent molecules, which are useful to enhance the biocompatibility of titanium surfaces.

Original languageEnglish (US)
Pages (from-to)5882-5887
Number of pages6
JournalApplied Surface Science
Issue number20
StatePublished - Aug 1 2010


  • Biocompatibility
  • Cell adherent molecules
  • Cell signaling
  • Dental implant
  • Gelatin nano gold composite
  • Titanium modification

ASJC Scopus subject areas

  • Surfaces, Coatings and Films


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