TY - JOUR
T1 - Modified titanium surface with gelatin nano gold composite increases osteoblast cell biocompatibility
AU - Lee, Young Hee
AU - Bhattarai, Govinda
AU - Aryal, Santosh
AU - Lee, Nan Hee
AU - Lee, Min Ho
AU - Kim, Tae Gun
AU - Jhee, Eun Chung
AU - Kim, Hak Yong
AU - Yi, Ho Keun
N1 - Funding Information:
This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (No. R01-2007-000-20488-0 ).
PY - 2010/8/1
Y1 - 2010/8/1
N2 - This study examined the gelatin nano gold (GnG) composite for surface modification of titanium in addition to insure biocompatibility on dental implants or biomaterials. The GnG composite was constructed by gelatin and hydrogen tetrachloroaurate in presence of reducing agent, sodium borohydrate (NabH4). The GnG composite was confirmed by UV-VIS spectroscopy and transmission electron microscopy (TEM). A dipping method was used to modify the titanium surface by GnG composite. Surface was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The MC-3T3 E1 cell viability was assessed by trypan blue and the expression of proteins to biocompatibility were analyzed by Western blotting. The GnG composite showed well dispersed character, the strong absorption at 530 nm, roughness, regular crystal and clear C, Na, Cl, P, and Au signals onto titanium. Further, this composite allowed MC-3T3 E1 growth and viability compared to gelatin and pure titanium. It induced ERK activation and the expression of cell adherent molecules, FAK and SPARC, and growth factor, VEGF. However, GnG decreased the level of SAPK/JNK. This shows that GnG composite coated titanium surfaces have a good biocompatibility for osteoblast growth and attachment than in intact by simple and versatile dipping method. Furthermore, it offers good communication between cell and implant surfaces by regulating cell signaling and adherent molecules, which are useful to enhance the biocompatibility of titanium surfaces.
AB - This study examined the gelatin nano gold (GnG) composite for surface modification of titanium in addition to insure biocompatibility on dental implants or biomaterials. The GnG composite was constructed by gelatin and hydrogen tetrachloroaurate in presence of reducing agent, sodium borohydrate (NabH4). The GnG composite was confirmed by UV-VIS spectroscopy and transmission electron microscopy (TEM). A dipping method was used to modify the titanium surface by GnG composite. Surface was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The MC-3T3 E1 cell viability was assessed by trypan blue and the expression of proteins to biocompatibility were analyzed by Western blotting. The GnG composite showed well dispersed character, the strong absorption at 530 nm, roughness, regular crystal and clear C, Na, Cl, P, and Au signals onto titanium. Further, this composite allowed MC-3T3 E1 growth and viability compared to gelatin and pure titanium. It induced ERK activation and the expression of cell adherent molecules, FAK and SPARC, and growth factor, VEGF. However, GnG decreased the level of SAPK/JNK. This shows that GnG composite coated titanium surfaces have a good biocompatibility for osteoblast growth and attachment than in intact by simple and versatile dipping method. Furthermore, it offers good communication between cell and implant surfaces by regulating cell signaling and adherent molecules, which are useful to enhance the biocompatibility of titanium surfaces.
KW - Biocompatibility
KW - Cell adherent molecules
KW - Cell signaling
KW - Dental implant
KW - Gelatin nano gold composite
KW - Titanium modification
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U2 - 10.1016/j.apsusc.2010.03.069
DO - 10.1016/j.apsusc.2010.03.069
M3 - Article
AN - SCOPUS:77953315459
SN - 0169-4332
VL - 256
SP - 5882
EP - 5887
JO - Applied Surface Science
JF - Applied Surface Science
IS - 20
ER -